KDM5B Protein (AA 2-751) (DYKDDDDK Tag)

Catalog No. ABIN7939249

This Recombinant KDM5B protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for KDM5B Protein (AA 2-751) (DYKDDDDK Tag) (ABIN7939249)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 2-751

Purification tag / Conjugate: This KDM5B protein is labelled with DYKDDDDK Tag.

Purpose: Recombinant JARID1B / KDM5B (2-751) protein

Characteristics: Recombinant JARID1B / KDM5B (2-751) (accession number NP_006609) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 100 kDa. The recombinant protein is ≥ 80 % pure by SDS- PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant JARID1B / KDM5B (2-751) protein was expressed in Sf9 cells and is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5 % glycerol, 0.04 % Triton X-100, 0.2 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: JARID1B & KDM5B

Background:

Protein Background: Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.

Short Description: Recombinant JARID1B / KDM5B (2-751) (accession number NP_006609) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 100 kDa. The recombinant protein is ≥ 80 % pure by SDS- PAGE.

Aliases: Lysine Demethylase 5B, Cancer/Testis Antigen 31, Retinoblastoma-Binding Protein 2 Homolog 1, Histone Demethylase JARID1B

Molecular Weight: 100 kDa

NCBI Accession: NP_006609

Pathways: Warburg Effect