MMLV Protein

Details for Product No. ABIN805476, Supplier: Log in to see
Protein Name
Origin
Mouse (Murine)
Source
Escherichia coli (E. coli)
Protein Type
Recombinant
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Characteristics MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb)
Sterility Sterile filtered
Background MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb)
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-HCl, 0.1 M NaCl, 0.1 % Triton X-100, 0.1 mM DTT, 0.01 mM EDTA and 50 % glycerol.
Handling Advice Please avoid freeze thaw cycles.
Storage -20 °C
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