Chaperonin GroEL (GroEL) Protein

Catalog No. ABIN934885

Product Details

Target: Chaperonin GroEL (GroEL)

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM

Characteristics: Purified recombinant E.coli GroEL protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Purity: > 95 % pure

Application Details

Application Notes: GroEL protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 105 mM NaCl, 5 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: Chaperonin GroEL (GroEL)

Alternative Name: GroEL (GroEL Products)

Synonyms: CPN60 Protein, GROEL Protein, HLD4 Protein, HSP-60 Protein, HSP60 Protein, HSP65 Protein, HuCHA60 Protein, SPG13 Protein, cpn60_1 Protein, heat shock protein family D (Hsp60) member 1 Protein, chaperonin GroEL Protein, chaperonin Protein, GroEL Protein, groEL Protein, 60 KDA CHAPERONIN 2 GROEL2 (PROTEIN CPN60-2) (GROEL PROTEIN 2) (65 KDA ANTIGEN) (HEAT SHOCK PROTEIN 65) (CELL WALL PROTEIN A) (ANTIGEN A) Protein, Cpn60; groEL Protein, molecular chaperone GroEL Protein, HSPD1 Protein, groEL1 Protein, groEL Protein, groEL2 Protein, APH_RS01055 Protein

Background: GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin(HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress conditions. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.
Alternative Names: mopA protein, 60 kDa chaperonin protein, groL protein, groEL protein, groEL protein protein, chaperonin groel, Protein Cpn60 protein

Molecular Weight: 57.3 kDa (548 AA)