PTPN1 Protein
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- Target See all PTPN1 Proteins
- PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
- Characteristics
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Purified recombinant Human PTP1 B protein
Expression System: E.coli
Bioactivity: 10,000units/mg of PTP1B
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Top Product
- Discover our top product PTPN1 Protein
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Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 268-406) protein (His tag)
PTPN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-321) (Active) proteinPTPN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-435) protein (GST tag)PTPN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 322-406) protein (GST tag)PTPN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (Active) protein (His tag)PTPN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-435) protein (Strep Tag)Crystallography grade PTPN1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-432) protein (Strep Tag)Crystallography grade PTPN1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (DYKDDDDK Tag)PTPN1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (Myc-DYKDDDDK Tag)PTPN1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- PTP1B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Comment
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One unit will hydrolyze 1 nM of p-nitrophenyl-phosphatate per minute at pH 7.4 at 37 °C using 10 mM of substrate
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 2 mM beta-mercaptoethanol, 1 mM EDTA.1 mMDTT, and 20 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
- Alternative Name
- PTP1B (PTPN1 Products)
- Synonyms
- PTPN1 Protein, PTP1B Protein, PTP-1B Protein, PTP-HA2 Protein, Ptp Protein, ptp1b Protein, wu:fk54h03 Protein, CPTP1 Protein, protein tyrosine phosphatase, non-receptor type 1 Protein, protein tyrosine phosphatase, non-receptor type 1 L homeolog Protein, PTPN1 Protein, Ptpn1 Protein, ptpn1 Protein, ptpn1.L Protein
- Background
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The protein coding region of the catalytic domain of PTP-1B was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
Alternative Names: PTPN1 protein, PTPB 1 protein, Protein Tyrosine Phosphatase Non Receptor Type-1, PTPB-1, Protein Tyrosine Phosphatase 1B protein, PTP1B protein, PTPB 1, PTP-1B protein, EC 3.1.3.48 protein, PTP1B, PTPB-1 protein, Protein tyrosine phosphatase non-receptor type 1 protein, Protein-tyrosine phosphatase1B protein, Tyrosine-protein phosphatase non-receptor type 1 protein - Molecular Weight
- 37.3 kDa (321 AA)
- Pathways
- TLR Signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling
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