PDGF-BB Homodimer (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 98 % pure
Active
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- Target
- PDGF-BB Homodimer
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
- Characteristics
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Purified recombinant Human PDGF BB protein
Expression System: E.coli
Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/mL. - Purity
- > 98 % pure
- Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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PDGF-BB Homodimer (Active) protein (His tag)
Origin: Human Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer (AA 82-190) (Active) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Supplied as a lyophilized powder.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Target
- PDGF-BB Homodimer
- Alternative Name
- PDGF BB
- Synonyms
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
- Background
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PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
Alternative Names: Osteosarcoma-derived Growth Factor protein, Glioma-derived growth factor protein, Platelet-Derived Growth Factor-BB protein, GDGF protein, ODGF protein - Molecular Weight
- 28.7 kDa
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