TGFA Protein
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- Target See all TGFA Proteins
- TGFA (Transforming Growth Factor, alpha (TGFA))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
- Characteristics
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Purified recombinant Human TGF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ? 0.2 ng/mL, corresponding to a specific activity of ? 5 x 10^6 units/mg. - Purity
- > 98 % pure
- Endotoxin Level
- < 0.1 ng per μg (1 EU/μg).
- Top Product
- Discover our top product TGFA Protein
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Transforming Growth Factor, alpha (TGFA) (AA 40-89) protein (Fc Tag)
TGFA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transforming Growth Factor, alpha (TGFA) (AA 39-88) protein (Fc Tag)TGFA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transforming Growth Factor, alpha (TGFA) (AA 40-89) protein (His tag)TGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Transforming Growth Factor, alpha (TGFA) (AA 1-159) protein (GST tag)TGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transforming Growth Factor, alpha (TGFA) (AA 42-89) protein (GST tag)TGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transforming Growth Factor, alpha (TGFA) (AA 24-160) protein (rho-1D4 tag)Crystallography grade TGFA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, alpha (TGFA) (AA 40-89) protein (rho-1D4 tag)Crystallography grade TGFA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, alpha (TGFA) (AA 24-159) protein (rho-1D4 tag)Crystallography grade TGFA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, alpha (TGFA) (AA 39-88) protein (rho-1D4 tag)Crystallography grade TGFA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Comment
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Biological activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is approx. 0.2 ng/mL, corresponding to a specific activity of approx. 5 x 10^6 units/mg.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL.
- Buffer
- Supplied lyophilized with no additives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Target
- TGFA (Transforming Growth Factor, alpha (TGFA))
- Alternative Name
- TGF alpha (TGFA Products)
- Synonyms
- TFGA Protein, LOC100009482 Protein, TGF alpha Protein, TGFA Protein, wa-1 Protein, wa1 Protein, RATTGFAA Protein, TGFAA Protein, NV15606 Protein, si:ch211-10e8.7 Protein, transforming growth factor alpha Protein, transforming growth factor alpha L homeolog Protein, transforming growth factor-alpha Protein, transforming growth factor, alpha Protein, TGFA Protein, TGF-A Protein, Tgfa Protein, tgfa.L Protein, LOC100121300 Protein, LOC100009150 Protein, tgfa Protein
- Background
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TGF alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF alpha induces transformation anchorage independence in cultured cells. Human, Mouse and rat TGF alpha are cross-species reactive. Recombinant human TGF alpha is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40 % sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.
Alternative Names: TGF-alpha protein, TGFa protein, Transforming Growth Factor alpha protein, TGF a protein, TGFalpha protein - Molecular Weight
- 5.5 kDa
- Pathways
- NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
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