TRAIL Protein

Catalog No. ABIN935821

Product Details

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

Characteristics: Purified recombinant Human TRAIL protein
Expression System: E.coli
Bioactivity: Assay#1:Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/mL. Assay#2:Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8 - 2.0 ng/mL.

Purity: > 98 % pure

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: Assay#1:Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/mL. Assay#2:Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8 - 2.0 ng/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with water to 0.5-1.0 mg/mL .

Buffer: Lyophilized from 10 mM Na3PO4, pH 7.2, with 110 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TRAIL (TNFSF10 Products)

Synonyms: APO2L Protein, Apo-2L Protein, CD253 Protein, TL2 Protein, TRAIL Protein, A330042I21Rik Protein, AI448571 Protein, APO-2L Protein, Ly81 Protein, Trail Protein, tl2 Protein, apo2l Protein, cd253 Protein, trail Protein, apo-2l Protein, trail1 Protein, xtrail1 Protein, tnfsf10l2 Protein, zgc:92320 Protein, TNLG6A Protein, TNF superfamily member 10 Protein, tumor necrosis factor (ligand) superfamily, member 10 Protein, tumor necrosis factor superfamily member 10 Protein, TNF superfamily member 10 S homeolog Protein, TNFSF10 Protein, Tnfsf10 Protein, tnfsf10.S Protein, tnfsf10 Protein

Background: TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic death domain, which activates the cell's apoptotic machinery.
Alternative Names: TL2 protein, Apo2 Ligand protein, TNF-related apoptosis-inducing Ligand protein, TNFSF10 protein

Molecular Weight: 19.6 kDa

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity