Chemokine (C-C Motif) Ligand 15 (CCL15) (Active) Protein

Details for Product No. ABIN988168
Protein Name
Origin
Human
32
Source
Escherichia coli (E. coli)
21
4
2
2
Biological Activity
Active
Options
Sequence QFTNDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY S
Characteristics Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Endotoxin Level Level Less than 1EU/µg of rHuMIP-5/CCL15 as determined by LAL method
Protein Name
Background CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1γ, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells. Synonym: MIP-5/CCL15, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.
Molecular Weight 10.1 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.
Restrictions For Research Use only
Format Lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage 4 °C