anti-Pig (Porcine) Adducin 2 (Beta) antibody for Western Blotting

Recommended Adducin 2 (Beta) Antibody (supplied by: Log in to see )

Antigen
Adducin 2 (Beta) (ADD2) Antibodies
  • si:dkey-202n14.5
  • ADDB
  • 2900072M03Rik
  • add97
  • beta-ADD
  • adducin 2
  • adducin 2 (beta)
  • ADD2
  • add2
  • Add2
Epitope
Middle Region
7
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
62
39
36
2
2
1
1
1
Host
Rabbit
60
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
45
24
13
12
9
6
4
1
1
1
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Catalog No. ABIN2784900
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1891523 IP WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0

General

Antigen Adducin 2 (Beta) (ADD2) Antibodies
Epitope Middle Region
(7), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(62), (39), (36), (2), (2), (1), (1), (1)
Host Rabbit
(60), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(45), (24), (13), (12), (9), (6), (4), (1), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN972930
Sequence VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ADD2

Target Details

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Antigen
Alternative Name ADD2 (ADD2 Antibody Abstract)
Background Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
Alias Symbols: ADDB
Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1,
Protein Size: 726
Molecular Weight 80 kDa
Gene ID 119
NCBI Accession NM_001617, NP_001608
UniProt P35612
Pathways Regulation of Actin Filament Polymerization

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 726 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Kato, Miyata, Tabara, Katsuya, Yanai, Hanada, Kamide, Nakura, Kohara, Takeuchi, Mano, Yasunami, Kimura, Kita, Ueshima, Nakayama, Soma, Hata, Fujioka, Kawano, Nakao, Sekine, Yoshida, Nakamura, Saruta, Ogihara, Sugano, Miki, Tomoike: "High-density association study and nomination of susceptibility genes for hypertension in the Japanese National Project." in: Human molecular genetics, Vol. 17, Issue 4, pp. 617-27, 2008

Images

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Supplier Images
Western Blotting (WB) image for anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (ABIN2784900) WB Suggested Anti-ADD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
 image for anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (ABIN2784900) anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (Image 2)