anti-Pig (Porcine) CAPZB antibody for ELISA

Recommended CAPZB Antibody (supplied by: Log in to see )

Antigen
Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB) Antibodies
  • CAPB
  • CAPPB
  • CAPZ
  • wu:fk65f06
  • zgc:66149
  • MGC52754
  • capzb
  • 1700120C01Rik
  • AI325129
  • CPB1
  • CPB2
  • CPbeat2
  • CPbeta1
  • CPbeta2
  • Cappb1
  • capping actin protein of muscle Z-line beta subunit
  • capping protein (actin filament) muscle Z-line, beta
  • F-actin-capping protein subunit beta
  • f-actin-capping protein subunit beta
  • capping protein (actin filament) muscle Z-line, beta L homeolog
  • CAPZB
  • capzb
  • AOR_1_1412174
  • MCYG_05913
  • VDBG_04956
  • MGYG_06053
  • PGTG_03178
  • Tsp_07516
  • capzb.L
  • Capzb
Epitope
C-Term
19
14
11
6
3
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
79
18
16
8
6
5
3
2
2
2
2
1
1
1
1
Host
Goat
49
17
14
1
Clonality
Polyclonal
Conjugate
This CAPZB antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
80
45
11
9
4
3
2
1
Options
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Available images

Catalog No. ABIN184616
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN5907201 ELISA EIA IHC WB Goat Log in to see Polyclonal 0
7 ABIN2561560 ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
4 ABIN293430 ELISA WB Goat AA 260-272 Log in to see Polyclonal 0
3.6761246 ABIN257077 ELISA WB Goat C-Term, Isoform beta 2 Log in to see Polyclonal 1

General

Antigen Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB) Antibodies
Epitope C-Term
(19), (14), (11), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(79), (18), (16), (8), (6), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Goat
(49), (17), (14), (1)
Clonality
Polyclonal
Conjugate This CAPZB antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application ELISA, Western Blotting (WB)
(80), (45), (11), (9), (4), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-CAPZB Antibody

Target Details CAPZB Application Details Handling References for anti-CAPZB antibody (ABIN184616) Images
Purpose CAPZB
Blocking Peptide Blocking peptide for this product available: ABIN370256
Sequence NDLVEALKRK QQC
Specificity This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence NDLVEALKRKQQC, from the C Terminus of the protein sequence according to NP_004921.1.
Plasmids, Primers & others

Target Details CAPZB

Product Details anti-CAPZB Antibody Application Details Handling References for anti-CAPZB antibody (ABIN184616) Images back to top
Antigen
Alternative Name CAPZB (CAPZB Antibody Abstract)
Background CAPZB, capping protein (actin filament) muscle Z-line, beta, CAPB, CAPZ, CAPPB, Cap Z, RP4-657E11.7, MGC104401, MGC129749, MGC129750, Cap Z, F-actin capping protein beta subunit
Gene ID 832
NCBI Accession NP_004921
Pathways Regulation of Actin Filament Polymerization

Application Details

Product Details anti-CAPZB Antibody Target Details CAPZB Handling References for anti-CAPZB antibody (ABIN184616) Images back to top
Application Notes

DS WB Results: Approx 30 kDa band observed in lysates of cell lines Jurkat, HeLa and NIH-3T3 and approx 28 kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 30.6 kDa according to Human NP_004921.1 and 31.3 kDa according to Mouse NP_001032850.1, and 30.6 kDa according to Rat NP_001005903.1 and Pig NP_001106915.1]. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Wang CY et al, J Biomed Sci. 2011 Feb 7,18:13. PMID: 21299884.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only

Handling

Product Details anti-CAPZB Antibody Target Details CAPZB Application Details References for anti-CAPZB antibody (ABIN184616) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-CAPZB antibody (ABIN184616)

Product Details anti-CAPZB Antibody Target Details CAPZB Application Details Handling Images back to top
Product cited in:

Wang, Chen, Wu, Tsai, Shyue, Yang, Tzeng: "Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury." in: Journal of biomedical science, Vol. 18, pp. 13, 2011

Background publications

Hart, Cooper: "Vertebrate isoforms of actin capping protein beta have distinct functions In vivo." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1287-98, 2000

Images

Product Details anti-CAPZB Antibody Target Details CAPZB Application Details Handling References for anti-CAPZB antibody (ABIN184616) back to top
Supplier Images
Western Blotting (WB) image for anti-Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB) (C-Term) antibody (ABIN184616) ABIN184616 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane...