anti-Guinea Pig Junction Mediating and Regulatory Protein, P53 Cofactor antibody for Western Blotting

Recommended Junction Mediating and Regulatory Protein, P53 Cofactor Antibody (supplied by: Log in to see )

Antigen
Junction Mediating and Regulatory Protein, P53 Cofactor (JMY) Antibodies
  • WHDC1L3
  • junction mediating and regulatory protein, p53 cofactor
  • junction-mediating and regulatory protein
  • JMY
  • jmy
  • Jmy
Epitope
N-Term
20
9
8
7
4
3
3
2
2
2
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
67
19
4
4
4
3
3
1
1
Host
Rabbit
47
20
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
57
40
23
8
4
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN2789961
$ 289.00
Plus shipping costs $45.00

General

Antigen Junction Mediating and Regulatory Protein, P53 Cofactor (JMY) Antibodies
Epitope N-Term
(20), (9), (8), (7), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
(67), (19), (4), (4), (4), (3), (3), (1), (1)
Host Rabbit
(47), (20)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(57), (40), (23), (8), (4), (2), (1), (1), (1), (1)
Supplier Log in to see

Product details

Target Details Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN978713
Sequence VFIVAWNEIE GKFAITCHNR TAQRQRSGSR EQAGARGGAE AGGAASDGSR
Predicted Reactivity Cow: 86%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against JMY. It was validated on Western Blot.
Purification Affinity Purified

Target Details

Product details Application Details Handling Images back to top
Antigen
Alternative Name JMY (JMY Antibody Abstract)
Background JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. JMY increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. JMY activates the Arp2/3 complex to induce branched actin filament networks. JMY also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. JMY contributes to cell motility by controlling actin dynamics. JMY may promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Alias Symbols: FLJ37870, MGC163496, WHDC1L3
Protein Interaction Partner: CCDC136, UBC, EP300, MDM2, E2F4, E2F3, E2F2, E2F1,
Protein Size: 712
Molecular Weight 82 kDa
Gene ID 133746
NCBI Accession NM_152405, NP_689618
UniProt Q8N9B5
Pathways Regulation of Actin Filament Polymerization

Application Details

Product details Target Details Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 712 AA

Restrictions For Research Use only

Handling

Product details Target Details Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product details Target Details Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Junction Mediating and Regulatory Protein, P53 Cofactor (JMY) (N-Term) antibody (ABIN2789961) WB Suggested Anti-JMY Antibody Titration: 1.0 ug/ml Positive Control: Fetal Lung