anti-Horse (Equine) NCK-Associated Protein 1-Like antibody for ELISA

Recommended NCK-Associated Protein 1-Like Antibody (supplied by: Log in to see )

Antigen
NCK-Associated Protein 1-Like (NCKAP1L) Antibodies
  • HEM1
  • 4930568P13Rik
  • AI463083
  • Hem1
  • Hemp1
  • NCK associated protein 1 like
  • zgc:172352
  • nck-associated protein 1-like
  • NCK-associated protein 1-like
  • NCKAP1L
  • zgc:172352
  • LOC100030968
  • nckap1l
  • LOC100618382
  • LOC100639088
  • Nckap1l
Epitope
Internal Region
8
4
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Horse (Equine)
55
34
26
10
8
7
6
4
3
3
2
1
1
Host
Goat
38
18
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
23
22
13
9
4
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Catalog No. ABIN308428
$ 291.46
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2563022 ELISA Goat IgG Internal Region Log in to see Polyclonal 0

General

Antigen NCK-Associated Protein 1-Like (NCKAP1L) Antibodies
Epitope Internal Region
(8), (4), (3), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Horse (Equine)
(55), (34), (26), (10), (8), (7), (6), (4), (3), (3), (2), (1), (1)
Host Goat
(38), (18)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Method Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(23), (22), (13), (9), (4)
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Product details

Target Details Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN368712
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-RHTENVTKTKTPE, from the internal region of the protein sequence according to NP_005328.2.

Target Details

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Antigen
Alternative Name HEM1 (NCKAP1L Antibody Abstract)
Background Synonyms: NCKAP1L, NCK-associated protein 1-like, HEM1, hematopoietic protein 1, membrane-associated protein hem-1
Gene ID 3071, 105855, 315348
NCBI Accession NP_005328
Pathways Regulation of Actin Filament Polymerization

Application Details

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Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx. 50 kDa band in Human Tonsil and Thymus lysates after 0.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128 kDa according to NP_005328.2. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only

Handling

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Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.