anti-Human Netrin 1 antibody for Immunofluorescence

Recommended Netrin 1 Antibody

Antigen
Netrin 1 (NTN1) Antibodies
  • ntn1-A
  • Netrin-1
  • NTN1
  • unc-6
  • MGC147073
  • NTN1L
  • AI561871
  • BC019633
  • netrin-1
  • netrin 1 L homeolog
  • netrin 1
  • ntn1.L
  • NTN1
  • ntn1
  • Ntn1
Reactivity
Human, Mouse (Murine)
93
79
62
10
10
6
4
4
3
2
2
1
1
Host
Chicken
79
14
10
5
4
2
1
Clonality
Polyclonal
Conjugate
This Netrin 1 antibody is un-conjugated
6
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
76
40
35
15
13
9
7
7
4
3
2
2
Options

Available images

Catalog No. ABIN953654
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
5.5 ABIN187960 IF IHC WB Chicken Polyclonal 1
4 ABIN2963562 ICC IF IHC Chicken Polyclonal 0
3.5383859 ABIN151152 ICC IF IHC IHC (fro) IHC (p) WB Chicken Polyclonal 6
-1.053978 ABIN4338688 ICC IF IHC IHC (p) Rabbit IgG Polyclonal 0

General

Antigen Netrin 1 (NTN1) Antibodies
Reactivity Human, Mouse (Murine)
(93), (79), (62), (10), (10), (6), (4), (4), (3), (2), (2), (1), (1)
Host Chicken
(79), (14), (10), (5), (4), (2), (1)
Clonality
Polyclonal
Conjugate This Netrin 1 antibody is un-conjugated
(6), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(76), (40), (35), (15), (13), (9), (7), (7), (4), (3), (2), (2)
Supplier

Product Details anti-Netrin 1 Antibody

Target Details Netrin 1 Application Details Handling Images
Specificity Recognizes Netrin-1.
Purification Affinity Chromatography using a peptide column.
Immunogen Synthetic peptide KLH conjugated corresponding to the netrin-1 gene product, but was shared between the Human (O95631) and Mouse (O9118) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Netrin 1

Product Details anti-Netrin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Netrin-1 (NTN1 Antibody Abstract)
Background Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Netrin 1's main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity. Murine Netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various nonneural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of "commissural neurons" from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.Synonyms: NTN1, NTN1L
Gene ID 9423
NCBI Accession NP_004813
Pathways Regulation of Cell Size

Application Details

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Images back to top
Concentration 0.1 mg/mL
Buffer 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted in the dark at 2-8 °C.

Images

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Netrin 1 (NTN1) antibody (ABIN953654) Dissociated cell cultures of an e13 Mouse brain showing Netrin-1 (green staining) in ...