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|Antigen||Calcium/calmodulin-Dependent Protein Kinase (CaM Kinase) II beta (CAMK2B) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This CAMK2B antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Product Details anti-CAMK2B AntibodyTarget Details CAMK2B Application Details Handling Images
|Specificity||Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).|
A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated
|Plasmids, Primers & others|
Target Details CAMK2BProduct Details anti-CAMK2B Antibody Application Details Handling Images back to top
|Alternative Name||CAMK2 / CAMKII (CAMK2B Antibody Abstract)|
Name/Gene ID: CAMK2
|Pathways||WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity|
Application DetailsProduct Details anti-CAMK2B Antibody Target Details CAMK2B Handling Images back to top
Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Target Species of Antibody: Rat
|Restrictions||For Research Use only|
HandlingProduct Details anti-CAMK2B Antibody Target Details CAMK2B Application Details Images back to top
|Buffer||BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)|
|Storage Comment||Store at -20°C.|
ImagesProduct Details anti-CAMK2B Antibody Target Details CAMK2B Application Details Handling back to top