anti-Rat (Rattus) Metabotropic Glutamate Receptor 5 antibody for Flow Cytometry

Recommended Metabotropic Glutamate Receptor 5 Antibody

Antigen
Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
  • GPRC1E
  • MGLUR5
  • mGlu5
  • mGluR5
  • mGlur5
  • 6430542K11Rik
  • AI850523
  • Glu5R
  • Gprc1e
  • mGluR5b
  • glutamate receptor, metabotropic 5
  • glutamate metabotropic receptor 5
  • GRM5
  • Grm5
Epitope
C-Term
82
70
15
14
11
7
4
3
3
2
2
2
2
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
199
155
145
7
7
5
5
3
3
3
2
2
1
Host
Rabbit
109
91
4
2
Clonality
Polyclonal
Conjugate
This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
9
7
7
6
6
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
165
113
83
69
58
37
36
22
13
4
3
3
2
1
Options

Available images

Catalog No. ABIN1574147
$ 160.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
16 ABIN4904353 FACS ICC IF IHC IP WB Rabbit IgG 0

General

Antigen Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
Epitope C-Term
(82), (70), (15), (14), (11), (7), (4), (3), (3), (2), (2), (2), (2), (1)
Reactivity Rat (Rattus), Mouse (Murine), Human
(199), (155), (145), (7), (7), (5), (5), (3), (3), (3), (2), (2), (1)
Host Rabbit
(109), (91), (4), (2)
Clonality
Polyclonal
Conjugate This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
(9), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(165), (113), (83), (69), (58), (37), (36), (22), (13), (4), (3), (3), (2), (1)
Supplier

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Target Details Metabotropic Glutamate Receptor 5 Application Details Handling Images
Specificity Rabbit Anti-mGluR5 Polyclonal Antibody detects endogenous levels of human mGluR5. It is predicted to react with mouse and rat mGluR5 protein according to sequence homology.Positive Control: Ramos
Cross-Reactivity (Details) Rabbit Anti-mGluR5 Polyclonal Antibody detects endogenous levels of human mGluR5. It is predicted to react with mouse and rat mGluR5 protein according to sequence homology.Positive Control: Ramos
Purification Immunoaffinity chromatography
Immunogen KLH-coupled synthetic peptide from C-terminal of human mGluR5
Isotype IgG
Plasmids, Primers & others

Target Details Metabotropic Glutamate Receptor 5

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name mGluR5 (GRM5 Antibody Abstract)
Background Metabotropic glutamate receptor 5 (mGluR5) is one member of G-protein coupled receptor 3 family. The activity of mGluR5 receptor is mediated by a G-protein that activates phosphatidylinositol-calcium second messenger system and generates calcium-activated chloride current. mGluR5 plays a critical role in the modulation of synaptic plasticity and behavioral responses to multiple substances of abuse. The expression of mGluR5 is widely distributed in brain regions relating to memory, such as the hippocampus. mGluR5 regulates phospholipid hydrolysis in developing cortical neurons through the activation of PLC-beta1. The receptor regulates glutamatergic transmission in a variety of brain regions, particularly those thought to participate in reward-related behaviors. Its activation by the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) can induce long term depression (DHPG-LTD) of glutamatergic synaptic transmission.Rabbit Anti-mGluR5 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human mGluR5 (Swiss Prot: P41594).
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Handling Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
Restrictions For Research Use only

Handling

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Images

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN1574147) Western blot analysis of cell lysate using 2 µg/mL Rabbit Anti-mGluR5 Polyclonal Anti...
Flow Cytometry (FACS) image for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN1574147) Flow cytometric analysis of Ramos cells using mGluR5 Antibody, pAb, Rabbit (ABIN39899...