anti-Human MGLL antibody for Western Blotting

Recommended MGLL Antibody

Antigen
Monoglyceride Lipase (MGLL) Antibodies
  • cb909
  • wu:fc66g01
  • zgc:56561
  • MGC84195
  • MGLL
  • mgl
  • hu-k5
  • mgll
  • HU-K5
  • HUK5
  • MAGL
  • MGL
  • AA589436
  • Magl
  • Mgl
  • monoglyceride lipase
  • monoglyceride lipase L homeolog
  • 13L protein
  • Monoglyceride lipase
  • mgll
  • MGLL
  • mgll.L
  • 13L
  • YMTVg13L
  • EHI_044210
  • EHI_045330
  • TATV_DAH68_039
  • Mgll
Epitope
Internal Region
13
10
7
7
6
6
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
137
39
34
5
4
3
2
1
1
1
1
1
1
Host
Goat
77
49
17
1
1
Clonality
Polyclonal
Conjugate
This MGLL antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
95
46
44
24
17
16
16
13
3
2
1
1
Options

Available images

Catalog No. ABIN334436
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
26.441288 ABIN2855646 IHC (p) WB Rabbit IgG Center Polyclonal 0
19 ABIN1499437 FACS IF IHC IHC (p) WB Mouse IgG2b 4D1 0
19 ABIN1499438 FACS IF IHC IHC (p) WB Mouse IgG1 1C6 0
19 ABIN1499436 FACS IF IHC IHC (p) WB Mouse IgG2a 2G8 0
19 ABIN1499442 IF IHC IHC (p) WB Mouse IgG1 1B1 0
17.441288 ABIN928632 WB Rabbit N-Term Polyclonal 0
13.550953 ABIN2146522 FACS IHC WB Mouse IgG2b 2C9 0
13.550953 ABIN2146523 FACS WB Mouse IgG1 2B6 0
13.550953 ABIN2146524 FACS IF IHC WB Mouse IgG2b 4D1 0
13.550953 ABIN2146525 FACS IHC WB Mouse IgG1 1A1 0
13.550953 ABIN2146526 FACS IF IHC WB Mouse IgG1 1C6 0
13.550953 ABIN2146529 IHC WB Mouse IgG1 1H2 0
13.550953 ABIN2146528 IF IHC WB Mouse IgG1 1B1 0
13.550953 ABIN2146527 FACS IF IHC WB Mouse IgG2a 2G8 0
13.550953 ABIN6143823 WB Rabbit Polyclonal 0
13 ABIN1499440 FACS IHC IHC (p) WB Mouse IgG1 1A1 0
10.890334 ABIN2726234 FACS IF IHC WB Mouse IgG2b 4D1 0
10.890334 ABIN2726235 IF IHC WB Mouse IgG1 1B1 0
10.890334 ABIN2726232 FACS IF IHC WB Mouse IgG2a 1H1 0
10.890334 ABIN2726226 FACS IF IHC WB Mouse IgG2a 2B11 0

General

Antigen Monoglyceride Lipase (MGLL) Antibodies
Epitope Internal Region
(13), (10), (7), (7), (6), (6), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(137), (39), (34), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(77), (49), (17), (1), (1)
Clonality
Polyclonal
Conjugate This MGLL antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(95), (46), (44), (24), (17), (16), (16), (13), (3), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-MGLL Antibody

Target Details MGLL Application Details Handling References for anti-MGLL antibody (ABIN334436) Images
Purpose Monoglyceride Lipase
Blocking Peptide Blocking peptide for this product available: ABIN369607
Sequence QDLPHLVNAD GQY
Specificity This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).
Predicted Reactivity Human, Mouse, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.
Plasmids, Primers & others

Target Details MGLL

Product Details anti-MGLL Antibody Application Details Handling References for anti-MGLL antibody (ABIN334436) Images back to top
Antigen
Alternative Name MGLL (MGLL Antibody Abstract)
Background Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
Gene ID 11343
NCBI Accession NP_009214, NP_001003794
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism

Application Details

Product Details anti-MGLL Antibody Target Details MGLL Handling References for anti-MGLL antibody (ABIN334436) Images back to top
Application Notes

DS WB Results: Approx 35 kDa band observed in Human Brain (Frontal Cortex) and Mouse Adipose lysates (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat for this particular batch.

DS IHC Results: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions For Research Use only

Handling

Product Details anti-MGLL Antibody Target Details MGLL Application Details References for anti-MGLL antibody (ABIN334436) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-MGLL antibody (ABIN334436)

Product Details anti-MGLL Antibody Target Details MGLL Application Details Handling Images back to top
Background publications

Blankman, Simon, Cravatt: "A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol." in: Chemistry & biology, Vol. 14, Issue 12, pp. 1347-56, 2007

Images

Product Details anti-MGLL Antibody Target Details MGLL Application Details Handling References for anti-MGLL antibody (ABIN334436) back to top
Supplier Images
 image for anti-Monoglyceride Lipase (MGLL) (Internal Region) antibody (ABIN334436) ABIN334436 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer)....
Western Blotting (WB) image for anti-Monoglyceride Lipase (MGLL) (Internal Region) antibody (ABIN334436) ABIN334436 (0.5µg/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysa...
Immunohistochemistry (IHC) image for anti-Monoglyceride Lipase (MGLL) (Internal Region) antibody (ABIN334436) ABIN334436 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen r...
Immunohistochemistry (IHC) image for anti-Monoglyceride Lipase (MGLL) (Internal Region) antibody (ABIN334436) ABIN334436 Negative Control showing staining of paraffin embedded Human Liver, with n...