anti-Chemical Rhodopsin antibody for Immunoprecipitation

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Reactivity
Chemical
156
60
53
53
39
36
36
35
23
22
21
18
18
16
4
2
2
2
2
1
1
Host
Mouse
238
74
5
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
15
14
13
13
12
10
9
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
294
218
178
163
152
133
59
40
3
2
1
1
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Catalog No. ABIN863085
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4350402 ELISA ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 1D4 1
1 ABIN2482252 ICC IF IP IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482253 ICC IF IP IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482260 ICC IF IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 4D2 0
1 ABIN2482258 ICC IF IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482259 ICC IF IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482262 ICC IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see 4D2 0
1 ABIN2482266 ICC IF IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 4D2 0
1 ABIN2482280 ICC IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see 1D4 0
1 ABIN2482285 ICC IF IP IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 1D4 0
1 ABIN2482284 ICC IF IP IHC ELISA WB PE Mouse IgG1 Log in to see 1D4 0
1 ABIN2482261 ICC IF IP IHC ELISA WB APC Mouse IgG1 Log in to see 4D2 0
1 ABIN2482272 ICC IF IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 1D4 0
1 ABIN2482283 ICC IF IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 1D4 0
1 ABIN2482254 ICC IF IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482271 ICC IF IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 1D4 0
1 ABIN2482255 ICC IF IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482256 ICC IF IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482257 ICC IF IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 4D2 0
1 ABIN2482263 ICC IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see 4D2 0

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Chemical
(156), (60), (53), (53), (39), (36), (36), (35), (23), (22), (21), (18), (18), (16), (4), (2), (2), (2), (2), (1), (1)
Host Mouse
(238), (74), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(15), (14), (13), (13), (12), (10), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(294), (218), (178), (163), (152), (133), (59), (40), (3), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN863085) Images
Specificity Detects ~40 kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Purification Protein G Purified
Immunogen Bovine Rhodopsin
Clone 1D4
Isotype IgG1
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin antibody (ABIN863085) Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Gene ID 509933
NCBI Accession NP_001014890
UniProt P02699
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin antibody (ABIN863085) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-177 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin antibody (ABIN863085) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-Rhodopsin antibody (ABIN863085)

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Product cited in:

Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, 2012

Background publications

Iannaccone, Man, Waseem, Jennings, Ganapathiraju, Gallaher, Reese, Bhattacharya, Klein-Seetharaman: "Retinitis pigmentosa associated with rhodopsin mutations: Correlation between phenotypic variability and molecular effects." in: Vision research, Vol. 46, Issue 27, pp. 4556-67, 2006

Ridge, Lee, Abdulaev: "Examining rhodopsin folding and assembly through expression of polypeptide fragments." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7860-7, 1996

Molday, Hicks, Molday: "Peripherin. A rim-specific membrane protein of rod outer segment discs." in: Investigative ophthalmology & visual science, Vol. 28, Issue 1, pp. 50-61, 1987

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN863085) back to top
Supplier Images
Western Blotting (WB) image for anti-Rhodopsin (RHO) antibody (ABIN863085) Rho Human Cell Line Mix, Western Blotting.