anti-Chemical Rhodopsin antibody for Immunoprecipitation

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Epitope
N-Term
19
17
11
7
6
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Chemical
95
58
55
54
53
50
50
41
40
39
36
23
18
16
4
2
2
2
2
1
1
Host
Mouse
217
73
5
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
15
14
12
9
9
9
6
6
6
6
6
6
6
6
6
6
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
273
205
164
140
128
124
42
40
5
1
1
1
1
Supplier
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Catalog No. ABIN5550990
$ 517.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5550991 EIA IF IHC (fro) IP WB Mouse IgG1 C-Term Log in to see 1D4 0
1 ABIN1721672 FACS IHC IP WB Mouse IgG1 Log in to see 4D2 0
1 ABIN1721671 ICC IP IHC WB Mouse IgG1 Log in to see 1D4 0
1 ABIN2476360 IHC (p) IP WB Mouse IgG1 Log in to see 1D4 4
1 ABIN2351372 IC IP IHC ELISA WB Mouse IgG1 Log in to see 4D2 0
-2.2582502 ABIN4350402 ELISA ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 1D4 1

General

Antigen Rhodopsin (RHO) Antibodies
Epitope N-Term
(19), (17), (11), (7), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chemical
(95), (58), (55), (54), (53), (50), (50), (41), (40), (39), (36), (23), (18), (16), (4), (2), (2), (2), (2), (1), (1)
Host Mouse
(217), (73), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(15), (14), (12), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(273), (205), (164), (140), (128), (124), (42), (40), (5), (1), (1), (1), (1)
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling Images
Specificity N-terminus of Rhodopsin.
Cross-Reactivity (Details) Species reactivity (tested):All Mammalian species (Human, Mouse, Rat, Bovine, Pig, etc), Most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
Characteristics Synonyms: RHO, Opsin-2, OPN2
Purification Protein G Chromatography.
Immunogen Bovine Rhodopsin.
Clone 4D2
Isotype IgG1
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Target Type Chemical
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO
Gene ID 6010
UniProt P08100
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top
Application Notes ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months