anti-Amphibian ARNT antibody for Gel Shift

Recommended ARNT Antibody (supplied by: Log in to see )

Antigen
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
  • HIF-1-beta
  • HIF-1beta
  • HIF1-beta
  • HIF1B
  • HIF1BETA
  • TANGO
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • Hif1b
  • W08714
  • mKIAA4051
  • Arnt1
  • ARNT
  • zgc:136664
  • arnt1
  • hif1b
  • tango
  • xArnt
  • hif1beta
  • hif-1beta
  • arnt.2
  • MGC68805
  • aryl hydrocarbon receptor nuclear translocator
  • aryl hydrocarbon receptor nuclear translocator S homeolog
  • ARNT
  • Arnt
  • arnt
  • arnt.S
Reactivity
Amphibian, Human, Mouse (Murine), Primate, Rat (Rattus), Zebrafish (Danio rerio)
163
75
68
32
26
21
18
8
8
7
7
6
6
2
2
1
Host
Mouse
84
59
28
Clonality
Monoclonal
Conjugate
This ARNT antibody is un-conjugated
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
147
67
62
46
42
19
19
10
7
7
6
5
3
3
2
1
1
Supplier
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Catalog No. ABIN152684
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General

Antigen Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
Reactivity Amphibian, Human, Mouse (Murine), Primate, Rat (Rattus), Zebrafish (Danio rerio)
(163), (75), (68), (32), (26), (21), (18), (8), (8), (7), (7), (6), (6), (2), (2), (1)
Host Mouse
(84), (59), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ARNT antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(147), (67), (62), (46), (42), (19), (19), (10), (7), (7), (6), (5), (3), (3), (2), (1), (1)
Pubmed 10 references available
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Product Details anti-ARNT Antibody

Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN152684) Images
Specificity Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
Purification Immunogen affinity purified
Immunogen Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Clone 2B10
Isotype IgG1
Plasmids, Primers & others

Target Details ARNT

Product Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN152684) Images back to top
Antigen
Alternative Name ARNT/HIF-1 beta (ARNT Antibody Abstract)
Background Gene Symbol: ARNT
Gene ID 405
UniProt P27540
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN152684) Images back to top
Application Notes Western Blot 1:500 - 1:3000, Immunohistochemistry 5 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 5 μg/mL, Gel Super Shift Assays 1:1 - 1:100May be useful in ELISA, IF, IP, Gel Super Shift Assays, and WB. ChIP usage was reported in scientific literature. WB: Detects an approx. 87 kDa protein representing ARNT in COS cell extract. IF: Staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using NB 300-525 yields non-nucleolar, nuclear staining only.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN152684) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS with 1.0 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-ARNT antibody (ABIN152684)

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top
Product cited in:

Mandl, Lieberum, Depping: "A HIF-1α-driven feed-forward loop augments HIF signalling in Hep3B cells by upregulation of ARNT." in: Cell death & disease, Vol. 7, Issue 6, pp. e2284, 2016 Method employed by authors: Western Blotting (WB)

Mandl, Lieberum, Dunst, Depping: "The expression level of the transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT) determines cellular survival after radiation treatment." in: Radiation oncology (London, England), Vol. 10, pp. 229, 2015 (Sample species: Human). Further details: Western Blotting

Thomas, Harding, Smith, Overdevest, Nitz, Frierson, Tomlins, Kristiansen, Theodorescu: "CD24 is an effector of HIF-1-driven primary tumor growth and metastasis." in: Cancer research, Vol. 72, Issue 21, pp. 5600-12, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Singh, Wyman, Casado, Garrett, Gasiewicz: "Treatment of mice with the Ah receptor agonist and human carcinogen dioxin results in altered numbers and function of hematopoietic stem cells." in: Carcinogenesis, Vol. 30, Issue 1, pp. 11-9, 2009

Amir, Wang, Simons, Mabjeesh: "SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11142-51, 2009

Hord, Perdew et al.: "Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear ..." in: Molecular pharmacology, Vol. 46, Issue 4, pp. 618-26, 1994

Mandl, Depping: "ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells." in: Cancer cell international, Vol. 17, pp. 77, 1970 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Background publications

Hirose, Morita, Ema, Mimura, Hamada, Fujii, Saijo, Gotoh, Sogawa, Fujii-Kuriyama: "cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt)." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1706-13, 1996

Vaziri, Schneider, Sherr, Faller: "Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 271, Issue 42, pp. 25921-7, 1996

Hoffman, Reyes, Chu, Sander, Conley, Brooks, Hankinson: "Cloning of a factor required for activity of the Ah (dioxin) receptor." in: Science (New York, N.Y.), Vol. 252, Issue 5008, pp. 954-8, 1991

Images

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN152684) back to top
Supplier Images
Western Blotting (WB) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN152684) Figure 1 shows a Western blot of ARNT on COS cell extract using ABIN152684.
Immunofluorescence (IF) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...