Get this product for free
Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
---|---|---|---|---|---|---|---|---|---|---|
14.060627 | ABIN151037 | ChIP GS ICC IF IHC IHC (p) IP SimWes WB | Rabbit | Log in to see | Polyclonal | 38 | ||||
10 | ABIN1721790 | ChIP GS IHC (p) IP IHC WB | Mouse | IgG1 kappa | AA 496-789 | Log in to see | H1beta234 | 0 |
General |
|
---|---|
Antigen | Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies |
Reactivity | Cow (Bovine), Ferret, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This ARNT antibody is un-conjugated Alternatives |
Application |
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
|
![]() |
91 references available |
Supplier | Log in to see |
Product Details anti-ARNT AntibodyTarget Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151055) Images |
|
Purification | Protein G purified |
Immunogen | Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540] |
Clone | H1beta234 |
Isotype | IgG1 kappa |
Plasmids, Primers & others | |
Target Details ARNTProduct Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN151055) Images back to top |
|
Antigen | |
Alternative Name | ARNT/HIF-1 beta (ARNT Antibody Abstract) |
Background | Gene Symbol: ARNT |
Molecular Weight | Theoretical MW: 92 kDa |
Gene ID | 405 |
UniProt | P27540 |
Pathways | Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2 |
Application DetailsProduct Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN151055) Images back to top |
|
Application Notes | Western Blot 1:500-1:2000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:100, Gel Super Shift AssaysThis HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25343232) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Protocol |
Protocol specific for HIF-1 beta Antibody Western Blot Procedure 1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6 % polyacrylamide gel. . Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol. . Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM). . Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5 % NFDM. . Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). . Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5 % NFDM. . Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes). . Drain membrane and place on saran wrap. . Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds. . Drain membrane and place on new saran wrap . Wrap up membrane and expose to film. . Develop accordingly.10X Western wash 24.2g Tris80g NaCl Tween-20 to 1 % pH 7.6 and QS to 4LStripping buffer100 mM BME2 % SDS62.5 mM Tris (pH 6.7)Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash. |
Restrictions | For Research Use only |
HandlingProduct Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN151055) Images back to top |
|
Format | Liquid |
Concentration | 1.4 mg/mL |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | -80 °C,-20 °C |
Storage Comment | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
References for anti-ARNT antibody (ABIN151055)Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top |
|
Product cited in: |
Samanta, Park, Ni, Li, Zahnow, Gabrielson, Pan, Semenza: "Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 6, pp. E1239-E1248, 2018 Murakami, Wang, Kalhorn, Schraml, Rathmell, Tan, Nemenoff, Stenmark, Jiang, Reyland, Heasley, Hu: "Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma." in: Oncogenesis, Vol. 6, Issue 1, pp. e287, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human). Lu, Chen, Shimoda, Park, Zhang, Tran, Zhang, Semenza: "Chemotherapy-Induced Ca(2+) Release Stimulates Breast Cancer Stem Cell Enrichment." in: Cell reports, Vol. 18, Issue 8, pp. 1946-1957, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human). Li, Heller, Bostick, Lee, Schjerven, Kastner, Chan, Chen, Zhou: "Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway." in: Immunity, Vol. 45, Issue 1, pp. 185-97, 2016 (Sample species: Human). Further details: Western Blotting Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." in: Nature, Vol. 537, Issue 7618, pp. 63-68, 2016 (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation Taylor, Bagnall, Mason, Levy, Fernig, See: "Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility." in: Open biology, Vol. 6, Issue 9, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence Altuntas, Rossin, Marsella, DEletto, Diaz-Hidalgo, Farrace, Campanella, Antonioli, Fimia, Piacentini: "The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation." in: Oncotarget, Vol. 6, Issue 42, pp. 44941-54, 2016 Method employed by authors: Western Blotting (WB) Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 Method employed by authors: Western Blotting (WB) Rossin, DEletto, Falasca, Sepe, Cocco, Fimia, Campanella, Mastroberardino, Farrace, Piacentini: "Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis." in: Cell death and differentiation, Vol. 22, Issue 3, pp. 408-18, 2015 Further details: Western Blotting Kim, Kim, Park, Jung, Cho, Kim, Han, Choi, Kwon: "NNC 55-0396, a T-type Ca2+ channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1α signal transduction." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 5, pp. 499-509, 2015 (Sample species: Human). Further details: Western Blotting Xiang, Gilkes, Hu, Luo, Bullen, Liang, Semenza: "HIF-1α and TAZ serve as reciprocal co-activators in human breast cancer cells." in: Oncotarget, Vol. 6, Issue 14, pp. 11768-78, 2015 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,Immunoprecipitation Su, Xin, Yin, Dyson, Coon, Farrow, Mestan, Ernst: "Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARNT)." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 1, pp. E30-40, 2015 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence Xiang, Gilkes, Hu, Takano, Luo, Lu, Bullen, Samanta, Liang, Semenza: "Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype." in: Oncotarget, Vol. 5, Issue 24, pp. 12509-27, 2015 (Sample species: Human). Further details: Chromatin Immunoprecipitation Frey, Stonko, Faugere, Riddle: "Hypoxia-inducible factor-1α restricts the anabolic actions of parathyroid hormone." in: Bone research, Vol. 2, pp. 14005, 2015 Method employed by authors: Immunofluorescence (IF) Sena, Wang, Pawlus, Hu: "HIFs enhance the transcriptional activation and splicing of adrenomedullin." in: Molecular cancer research : MCR, Vol. 12, Issue 5, pp. 728-41, 2014 (Sample species: Human). Further details: Western Blotting Wang, Gilkes, Takano, Xiang, Luo, Bishop, Chaturvedi, Green, Semenza: "Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 31, pp. E3234-42, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation Peng, Yuan, Khan, Nanduri, Makarenko, Reddy, Vasavda, Kumar, Semenza, Prabhakar: "Regulation of hypoxia-inducible factor-α isoforms and redox state by carotid body neural activity in rats." in: The Journal of physiology, Vol. 592, Issue 17, pp. 3841-58, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting Pawlus, Wang, Hu: "STAT3 and HIF1α cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells." in: Oncogene, Vol. 33, Issue 13, pp. 1670-9, 2014 (Sample species: Human). Further details: Western Blotting Xiang, Gilkes, Chaturvedi, Luo, Hu, Takano, Liang, Semenza: "Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 2, pp. 151-64, 2014 (Sample species: Human). Further details: Western Blotting Gilkes, Xiang, Lee, Chaturvedi, Hubbi, Wirtz, Semenza: "Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 3, pp. E384-93, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation |
ImagesProduct Details anti-ARNT Antibody Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151055) back to top |
|
Supplier Images |