|Antigen||Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies|
|Reactivity||Cow (Bovine), Ferret, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This ARNT antibody is un-conjugated Alternatives|
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
|34 references available|
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Product Details anti-ARNT AntibodyTarget Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151037) Images
|Specificity||This is specific for HIF-1 beta/ARNT. It is not known if NB100-110 cross-reacts with ARNT2 which is related to HIF-1 beta/ARNT but is the product of a different gene.|
|Immunogen||Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]|
|Plasmids, Primers & others|
Target Details ARNTProduct Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN151037) Images back to top
|Alternative Name||ARNT/HIF-1 beta (ARNT Antibody Abstract)|
|Background||Gene Symbol: ARNT|
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN151037) Images back to top
|Application Notes||Western Blot 1:2000, Simple Western 1:1000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:150, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:150, Gel Supershift AssayThis HIF-1 beta antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. This antibody did not work in Immunocytochemistry. By Western blot, this antibody recognizes a band at ~92 kDa representing HIF-1 beta in induced tissues and cells. Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations. Lower dilutions may be needed for whole cell extract preps. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 17003483). Use in Gel supper shift assay reported in scientific literature (PMID 11046109). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for HIF-1 beta Antibody Procedure Guide for NB100-110 (Polyclonal anti-HIF-1 beta antibody)Western Blot Procedure
1. Resolve aliquots (15 mg) of induced * nuclear protein extracts on a SDS/6 % polyacrylamide gel.
. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol.
. Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM).
. Incubate membranes for 1.5 hours at room temperature (RT) in NB100-110 diluted 1:2,000 ** in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Incubate membranes with HRP conjugated anti-Rabbit IgG for 1 hour (RT) in 1X western wash/5 % NFDM. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Drain membrane and place on saran wrap.
. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds.
. Drain membrane and place on new saran wrap
. Wrap up membrane and expose to film.
. Develop accordingly.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN151037) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-ARNT antibody (ABIN151037)Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top
|Product cited in:||
Grampp, Schmid, Salama, Lauer, Kranz, Platt, Smythies, Choudhry, Goppelt-Struebe, Ratcliffe, Mole, Schödel: "Multiple renal cancer susceptibility polymorphisms modulate the HIF pathway." in: PLoS genetics, Vol. 13, Issue 7, pp. e1006872, 2017 Method employed by authors: Chromatin Immunoprecipitation (ChIP) (Sample species: Human).
Kobayashi, Morinibu, Koyasu, Goto, Hiraoka, Harada: "A circadian clock gene, PER2, activates HIF-1 as an effector molecule for recruitment of HIF-1α to promoter regions of its downstream genes." in: The FEBS journal, Vol. 284, Issue 22, pp. 3804-3816, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Platt, Salama, Smythies, Choudhry, Davies, Hughes, Ratcliffe, Mole: "Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters." in: EMBO reports, Vol. 17, Issue 10, pp. 1410-1421, 2016 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Kraus, Grampp, Goppelt-Struebe, Schreiber, Kunzelmann, Peters, Leipziger, Schley, Schödel, Eckardt, Buchholz: "P2Y2R is a direct target of HIF-1α and mediates secretion-dependent cyst growth of renal cyst-forming epithelial cells." in: Purinergic signalling, Vol. 12, Issue 4, pp. 687-695, 2016 Further details: Chromatin Immunoprecipitation
Grampp, Platt, Lauer, Salama, Kranz, Neumann, Wach, Stöhr, Hartmann, Eckardt, Ratcliffe, Mole, Schödel: "Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF binding to a MYC enhancer." in: Nature communications, Vol. 7, pp. 13183, 2016 Method employed by authors: Western Blotting (WB)
Rataj, Möller, Jähne, Hönscheid, Zierau, Vollmer, Kretzschmar: "Progesterone, as well as 17β-estradiol, is important for regulating AHR battery homoeostasis in the rat uterus." in: Archives of toxicology, Vol. 89, Issue 3, pp. 393-404, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Wong, Richardson, Seykora, Cotsarelis, Simon: "Hypoxia-inducible factors regulate filaggrin expression and epidermal barrier function." in: The Journal of investigative dermatology, Vol. 135, Issue 2, pp. 454-61, 2015 (Sample species: Mouse (Murine)).
Salama, Masson, Simpson, Sciesielski, Sun, Tian, Ratcliffe, Mole: "Heterogeneous Effects of Direct Hypoxia Pathway Activation in Kidney Cancer." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134645, 2015 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Zhu, Jiang, Chehab: "FoxO4 interacts with the sterol regulatory factor SREBP2 and the hypoxia inducible factor HIF2α at the CYP51 promoter." in: Journal of lipid research, Vol. 55, Issue 3, pp. 431-42, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting
Dela Cruz, Schmidt-Kastner, Stevens, Steinbusch, Rutten: "Differential distribution of hypoxia-inducible factor 1-beta (ARNT or ARNT2) in mouse substantia nigra and ventral tegmental area." in: Journal of chemical neuroanatomy, Vol. 61-62, pp. 64-71, 2014 (Sample species: Mouse (Murine)).
Pawlus, Wang, Hu: "STAT3 and HIF1α cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells." in: Oncogene, Vol. 33, Issue 13, pp. 1670-9, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Sena, Wang, Hu: "BRG1 and BRM chromatin-remodeling complexes regulate the hypoxia response by acting as coactivators for a subset of hypoxia-inducible transcription factor target genes." in: Molecular and cellular biology, Vol. 33, Issue 19, pp. 3849-63, 2013 Further details: Immunoprecipitation
Koizume, Ito, Miyagi, Hirahara, Nakamura, Sakuma, Osaka, Takano, Ruf, Miyagi: "HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells." in: Nucleic acids research, Vol. 40, Issue 12, pp. 5389-401, 2012 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation
Schödel, Bardella, Sciesielski, Brown, Pugh, Buckle, Tomlinson, Ratcliffe, Mole: "Common genetic variants at the 11q13.3 renal cancer susceptibility locus influence binding of HIF to an enhancer of cyclin D1 expression." in: Nature genetics, Vol. 44, Issue 4, pp. 420-5, S1-2, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Lo, Matthews: "High-resolution genome-wide mapping of AHR and ARNT binding sites by ChIP-Seq." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 130, Issue 2, pp. 349-61, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Schito, Rey, Tafani, Zhang, Wong, Russo, Russo, Semenza: "Hypoxia-inducible factor 1-dependent expression of platelet-derived growth factor B promotes lymphatic metastasis of hypoxic breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 40, pp. E2707-16, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Glover, Irizarry, Scully, Campbell, Bowers, Aherne, Kominsky, MacManus, Colgan: "IFN-γ attenuates hypoxia-inducible factor (HIF) activity in intestinal epithelial cells through transcriptional repression of HIF-1β." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 3, pp. 1790-8, 2011 (Sample species: Human). Further details: Western Blotting
Schödel, Oikonomopoulos, Ragoussis, Pugh, Ratcliffe, Mole: "High-resolution genome-wide mapping of HIF-binding sites by ChIP-seq." in: Blood, Vol. 117, Issue 23, pp. e207-17, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Lin, Cong, Yun: "Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha." in: Molecular cancer research : MCR, Vol. 9, Issue 6, pp. 757-65, 2011 (Sample species: Human). Further details: Western Blotting
Stejskalova, Vecerova, Peréz, Vrzal, Dvorak, Nachtigal, Pavek: "Aryl hydrocarbon receptor and aryl hydrocarbon nuclear translocator expression in human and rat placentas and transcription activity in human trophoblast cultures." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 123, Issue 1, pp. 26-36, 2011 Further details: Immunohistochemistry (Paraffin-embedded Sections)
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