anti-Cow (Bovine) ARNT antibody for Simple Western

Recommended ARNT Antibody (supplied by: Log in to see )

Antigen
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
  • HIF-1-beta
  • HIF-1beta
  • HIF1-beta
  • HIF1B
  • HIF1BETA
  • TANGO
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • Hif1b
  • W08714
  • mKIAA4051
  • Arnt1
  • ARNT
  • zgc:136664
  • arnt1
  • hif1b
  • tango
  • xArnt
  • hif1beta
  • hif-1beta
  • arnt.2
  • MGC68805
  • aryl hydrocarbon receptor nuclear translocator
  • aryl hydrocarbon receptor nuclear translocator S homeolog
  • ARNT
  • Arnt
  • arnt
  • arnt.S
Reactivity
Cow (Bovine), Ferret, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
169
81
73
30
25
20
19
8
8
7
6
6
5
3
2
1
1
Host
Rabbit
89
61
28
Clonality
Polyclonal
Conjugate
This ARNT antibody is un-conjugated
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
154
66
65
49
46
21
19
10
7
6
6
5
3
3
2
1
Supplier
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Catalog No. ABIN151037
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General

Antigen Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
Reactivity Cow (Bovine), Ferret, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(169), (81), (73), (30), (25), (20), (19), (8), (8), (7), (6), (6), (5), (3), (2), (1), (1)
Host Rabbit
(89), (61), (28)
Clonality
Polyclonal
Conjugate This ARNT antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(154), (66), (65), (49), (46), (21), (19), (10), (7), (6), (6), (5), (3), (3), (2), (1)
Pubmed 34 references available
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Product Details anti-ARNT Antibody

Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151037) Images
Specificity This is specific for HIF-1 beta/ARNT. It is not known if NB100-110 cross-reacts with ARNT2 which is related to HIF-1 beta/ARNT but is the product of a different gene.
Purification Unpurified
Immunogen Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]
Plasmids, Primers & others

Target Details ARNT

Product Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN151037) Images back to top
Antigen
Alternative Name ARNT/HIF-1 beta (ARNT Antibody Abstract)
Background Gene Symbol: ARNT
Gene ID 405
UniProt P27540
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN151037) Images back to top
Application Notes Western Blot 1:2000, Simple Western 1:1000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:150, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:150, Gel Supershift AssayThis HIF-1 beta antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. This antibody did not work in Immunocytochemistry. By Western blot, this antibody recognizes a band at ~92 kDa representing HIF-1 beta in induced tissues and cells. Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations. Lower dilutions may be needed for whole cell extract preps. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 17003483). Use in Gel supper shift assay reported in scientific literature (PMID 11046109). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF-1 beta Antibody Procedure Guide for NB100-110 (Polyclonal anti-HIF-1 beta antibody)Western Blot Procedure
1. Resolve aliquots (15 mg) of induced * nuclear protein extracts on a SDS/6 % polyacrylamide gel.
. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol.
. Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM).
. Incubate membranes for 1.5 hours at room temperature (RT) in NB100-110 diluted 1:2,000 ** in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Incubate membranes with HRP conjugated anti-Rabbit IgG for 1 hour (RT) in 1X western wash/5 % NFDM. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Drain membrane and place on saran wrap.
. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds.
. Drain membrane and place on new saran wrap
. Wrap up membrane and expose to film.
. Develop accordingly.
Restrictions For Research Use only

Handling

Product Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN151037) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-ARNT antibody (ABIN151037)

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top
Product cited in:

Grampp, Schmid, Salama, Lauer, Kranz, Platt, Smythies, Choudhry, Goppelt-Struebe, Ratcliffe, Mole, Schödel: "Multiple renal cancer susceptibility polymorphisms modulate the HIF pathway." in: PLoS genetics, Vol. 13, Issue 7, pp. e1006872, 2017 Method employed by authors: Chromatin Immunoprecipitation (ChIP) (Sample species: Human).

Kobayashi, Morinibu, Koyasu, Goto, Hiraoka, Harada: "A circadian clock gene, PER2, activates HIF-1 as an effector molecule for recruitment of HIF-1α to promoter regions of its downstream genes." in: The FEBS journal, Vol. 284, Issue 22, pp. 3804-3816, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Platt, Salama, Smythies, Choudhry, Davies, Hughes, Ratcliffe, Mole: "Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters." in: EMBO reports, Vol. 17, Issue 10, pp. 1410-1421, 2016 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Kraus, Grampp, Goppelt-Struebe, Schreiber, Kunzelmann, Peters, Leipziger, Schley, Schödel, Eckardt, Buchholz: "P2Y2R is a direct target of HIF-1α and mediates secretion-dependent cyst growth of renal cyst-forming epithelial cells." in: Purinergic signalling, Vol. 12, Issue 4, pp. 687-695, 2016 Further details: Chromatin Immunoprecipitation

Grampp, Platt, Lauer, Salama, Kranz, Neumann, Wach, Stöhr, Hartmann, Eckardt, Ratcliffe, Mole, Schödel: "Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF binding to a MYC enhancer." in: Nature communications, Vol. 7, pp. 13183, 2016 Method employed by authors: Western Blotting (WB)

Rataj, Möller, Jähne, Hönscheid, Zierau, Vollmer, Kretzschmar: "Progesterone, as well as 17β-estradiol, is important for regulating AHR battery homoeostasis in the rat uterus." in: Archives of toxicology, Vol. 89, Issue 3, pp. 393-404, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wong, Richardson, Seykora, Cotsarelis, Simon: "Hypoxia-inducible factors regulate filaggrin expression and epidermal barrier function." in: The Journal of investigative dermatology, Vol. 135, Issue 2, pp. 454-61, 2015 (Sample species: Mouse (Murine)).

Salama, Masson, Simpson, Sciesielski, Sun, Tian, Ratcliffe, Mole: "Heterogeneous Effects of Direct Hypoxia Pathway Activation in Kidney Cancer." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134645, 2015 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Zhu, Jiang, Chehab: "FoxO4 interacts with the sterol regulatory factor SREBP2 and the hypoxia inducible factor HIF2α at the CYP51 promoter." in: Journal of lipid research, Vol. 55, Issue 3, pp. 431-42, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Dela Cruz, Schmidt-Kastner, Stevens, Steinbusch, Rutten: "Differential distribution of hypoxia-inducible factor 1-beta (ARNT or ARNT2) in mouse substantia nigra and ventral tegmental area." in: Journal of chemical neuroanatomy, Vol. 61-62, pp. 64-71, 2014 (Sample species: Mouse (Murine)).

Pawlus, Wang, Hu: "STAT3 and HIF1α cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells." in: Oncogene, Vol. 33, Issue 13, pp. 1670-9, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Sena, Wang, Hu: "BRG1 and BRM chromatin-remodeling complexes regulate the hypoxia response by acting as coactivators for a subset of hypoxia-inducible transcription factor target genes." in: Molecular and cellular biology, Vol. 33, Issue 19, pp. 3849-63, 2013 Further details: Immunoprecipitation

Koizume, Ito, Miyagi, Hirahara, Nakamura, Sakuma, Osaka, Takano, Ruf, Miyagi: "HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells." in: Nucleic acids research, Vol. 40, Issue 12, pp. 5389-401, 2012 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation

Schödel, Bardella, Sciesielski, Brown, Pugh, Buckle, Tomlinson, Ratcliffe, Mole: "Common genetic variants at the 11q13.3 renal cancer susceptibility locus influence binding of HIF to an enhancer of cyclin D1 expression." in: Nature genetics, Vol. 44, Issue 4, pp. 420-5, S1-2, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Lo, Matthews: "High-resolution genome-wide mapping of AHR and ARNT binding sites by ChIP-Seq." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 130, Issue 2, pp. 349-61, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Schito, Rey, Tafani, Zhang, Wong, Russo, Russo, Semenza: "Hypoxia-inducible factor 1-dependent expression of platelet-derived growth factor B promotes lymphatic metastasis of hypoxic breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 40, pp. E2707-16, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Glover, Irizarry, Scully, Campbell, Bowers, Aherne, Kominsky, MacManus, Colgan: "IFN-γ attenuates hypoxia-inducible factor (HIF) activity in intestinal epithelial cells through transcriptional repression of HIF-1β." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 3, pp. 1790-8, 2011 (Sample species: Human). Further details: Western Blotting

Schödel, Oikonomopoulos, Ragoussis, Pugh, Ratcliffe, Mole: "High-resolution genome-wide mapping of HIF-binding sites by ChIP-seq." in: Blood, Vol. 117, Issue 23, pp. e207-17, 2011 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Lin, Cong, Yun: "Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha." in: Molecular cancer research : MCR, Vol. 9, Issue 6, pp. 757-65, 2011 (Sample species: Human). Further details: Western Blotting

Stejskalova, Vecerova, Peréz, Vrzal, Dvorak, Nachtigal, Pavek: "Aryl hydrocarbon receptor and aryl hydrocarbon nuclear translocator expression in human and rat placentas and transcription activity in human trophoblast cultures." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 123, Issue 1, pp. 26-36, 2011 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151037) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN151037) Staining of skin, epidermis using ABIN151037.
Western Blotting (WB) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN151037) Western Blot: ARNT/HIF-1 beta Antibody [ABIN151037] - WB analysis of HIF-1 beta in MC...
Simple Western (SimWes) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN151037) Simple Western: ARNT/HIF-1 beta Antibody [ABIN151037] - Simple Western lane view show...