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|Antigen||Bone Morphogenetic Protein 2 (BMP2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BMP2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-BMP2 AntibodyTarget Details BMP2 Application Details Handling Images
E.coli derived recombinant Human BMP-2.
Type of Immunogen: Cell extract
Target Details BMP2Product Details anti-BMP2 Antibody Application Details Handling Images back to top
|Alternative Name||BMP2 / BMP-2 (BMP2 Antibody Abstract)|
Name/Gene ID: BMP2
Family: TGF beta
Synonyms: BMP2, BDA2, BMP-2, BMP-2A, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-BMP2 Antibody Target Details BMP2 Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC-P (5 μg/mL), Neut (1.3 - 1.9 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7430 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBMP-2 (200 ng/mL), a concentration of 1.3-1.9 μg/mL of this antibody is required. ELISA: To detect hBMP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-2 (LS-C104469) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Reconstitution||Reconstitute in sterile water|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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