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|Antigen||Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A) Antibodies|
|Epitope||AA 865-881, Intracellular Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CACNA1A antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CACNA1A AntibodyTarget Details CACNA1A Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) Images
|Cross-Reactivity (Details)||Mouse - AA 16-17 residues identical, human - AA 16-17 residues identical.|
Confirmed by mass-spectrography and amino acid analysis.
Antibody concentration after reconstitution:
1 µg peptide per 10 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino acid residues 865-881 of rat CaV2.1. Intracellular loop between domains II and III.|
Target Details CACNA1AProduct Details anti-CACNA1A Antibody Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) Images back to top
|Alternative Name||CaV2.1 (CACNA1A Antibody Abstract)|
Voltage dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation. The voltage dependent calcium channels are composed of several subunits, alpha1, beta, alpha2δ and gamma. Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type. The Cav2.1 (formally named alpha1A) makes up the alpha1 poreforming subunit in P/Q type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with Cav2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release. Mutations in the Cav2.1 have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6). The involvement of Cav2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA, a specific blocker of the Cav2.1 channel. The blocking sensitivity is dependent on the a subunit isoform and on the splice variant.
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process|
Application DetailsProduct Details anti-CACNA1A Antibody Target Details CACNA1A Handling References for anti-CACNA1A antibody (ABIN1742170) Images back to top
WB: approx. 1:200,
IHC: approx. 1:100
|Restrictions||For Research Use only|
HandlingProduct Details anti-CACNA1A Antibody Target Details CACNA1A Application Details References for anti-CACNA1A antibody (ABIN1742170) Images back to top
|Reconstitution||0.2 mL deionized water.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, it is recommended to add glycerol at a ratio of 1:1 and to keep the antibody solution at -20 °C.|
References for anti-CACNA1A antibody (ABIN1742170)Product Details anti-CACNA1A Antibody Target Details CACNA1A Application Details Handling Images back to top
Scheuber, Miles, Poncer: "Presynaptic Cav2.1 and Cav2.2 differentially influence release dynamics at hippocampal excitatory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 46, pp. 10402-9, 2004
Tottene, Fellin, Pagnutti, Luvisetto, Striessnig, Fletcher, Pietrobon: "Familial hemiplegic migraine mutations increase Ca(2+) influx through single human CaV2.1 channels and decrease maximal CaV2.1 current density in neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 13284-9, 2002
Bourinet, Soong, Sutton, Slaymaker, Mathews, Monteil, Zamponi, Nargeot, Snutch: "Splicing of alpha 1A subunit gene generates phenotypic variants of P- and Q-type calcium channels." in: Nature neuroscience, Vol. 2, Issue 5, pp. 407-15, 1999
ImagesProduct Details anti-CACNA1A Antibody Target Details CACNA1A Application Details Handling References for anti-CACNA1A antibody (ABIN1742170) back to top