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|Antigen||Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies|
|Epitope||AA 100-150 Alternatives|
|Reactivity||Dog (Canine), Human, Macaque, Monkey, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody recognizes Amine Oxidase (Flavin Containing) Domain 2 (AOF2/KDM1A).|
Species reactivity (expected):Dog (Canine), Macaque, Monkey, Mouse, Rat and Zebrafish.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to a portion of amino acids of human AOF2.|
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|Alternative Name||AOF2 (KDM1A Antibody Abstract)|
|Background||LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.Synonyms: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1|
|Molecular Weight||93 kDa.|
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling|
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Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 0.5-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
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