anti-Mammalian Lysine (K)-Specific Demethylase 1A antibody for Immunoprecipitation

Recommended Lysine (K)-Specific Demethylase 1A Antibody (supplied by: Log in to see )

Antigen
Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Reactivity
Human, Mammalian
212
122
93
21
20
10
8
6
5
4
3
1
1
1
Host
Rabbit
161
48
5
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
180
77
70
49
32
26
19
14
10
9
7
6
6
3
1
1
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Catalog No. ABIN577699
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN5582777 IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
Reactivity Human, Mammalian
(212), (122), (93), (21), (20), (10), (8), (6), (5), (4), (3), (1), (1), (1)
Host Rabbit
(161), (48), (5)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(180), (77), (70), (49), (32), (26), (19), (14), (10), (9), (7), (6), (6), (3), (1), (1)
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Product details

Target Details Application Details Handling Images
Brand AbX™
Characteristics Reacts with mammalian Lysine Specific Demethylase 1
Purification Purified
Immunogen Full length recombinant Lysine Specific Demethylase 1

Target Details

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Antigen
Alternative Name Lysine Specific Demethylase 1 (LSD1) (KDM1A Antibody Abstract)
Background There are two known classes of histone demethylases (HDM), differing in their reaction chemistry, coenzyme use and reaction products. Flavin-dependent HDMs act only on mono- and dimethylated lysines and produce hydrogen peroxide, whereas the Jumonji-containing HDMs are iron-dependent enzymes that can act on mono-, di- and trimethylated lysine side chains, and also on methylated Arginine residues. Both classes also produce formaldehyde as a product of the demethylation reaction. LSD1 (Lysine-specific demethylase 1) is a flavin- dependent amine oxidase and was identified as a subunit of different complexes and has been shown to be involved in transcriptional repression of genes through the demethylation of monomethyl Lys4 and dimethyl Lys4 Histone H3. LSD1 is also involved in transcriptional activation through the demethylation of monomethyl Lys9 and dimethyl Lys9 Histone H3. It is also able to demethylate both mono- and dimethyl Lys370 in the regulatory domain of the tumor suppressor p53.
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling

Application Details

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Application Notes Western blotting and Immunoprecipitation
Western blotting, 1:50-1:200 AbX™ Anti-LSD1 Rabbit pAb
Detection of LSD1 in nuclear extract of 20 μg of HeLa cells probed with A003-100UL at a 1:200 dilution. MW of LSD1 is 110 kDa.
Comment

Reacts with mammalian Lysine Specific Demethylase 1 Supplied as a PBS solution Applications include Western blotting and Immunoprecipitation

Restrictions For Research Use only

Handling

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Format Liquid
Buffer Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Storage Comment Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Images

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Supplier Images
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN577699) anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody