anti-Mouse (Murine) Lysine (K)-Specific Demethylase 1A antibody for Enzyme Immunoassay

Recommended Lysine (K)-Specific Demethylase 1A Antibody (supplied by: Log in to see )

Antigen
Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Epitope
N-Term
18
11
9
9
8
8
7
7
4
4
4
3
3
3
3
2
2
2
2
2
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
215
124
95
23
21
10
9
6
6
4
3
3
1
1
1
Host
Rabbit
162
51
5
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
185
81
72
48
32
26
20
14
10
9
7
6
5
3
1
1
1
Supplier
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Catalog No. ABIN5554975
$ 474.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554976 EIA IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN5552000 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 0
1 ABIN1105388 EIA IHC (p) WB Mouse IgG1 Log in to see 1B2E5 0

General

Antigen Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
Epitope N-Term
(18), (11), (9), (9), (8), (8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(215), (124), (95), (23), (21), (10), (9), (6), (6), (4), (3), (3), (1), (1), (1)
Host Rabbit
(162), (51), (5)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(185), (81), (72), (48), (32), (26), (20), (14), (10), (9), (7), (6), (5), (3), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Specificity This antibody detects LSD1 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1.
Isotype IgG

Target Details

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Antigen
Alternative Name AOF2 (KDM1A Antibody Abstract)
Background Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.Synonyms: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1
Gene ID 23028
NCBI Accession NP_055828
UniProt O60341
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling

Application Details

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Application Notes ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.