anti-Primate Lysine (K)-Specific Demethylase 1A antibody for Chromatin Immunoprecipitation

Recommended Lysine (K)-Specific Demethylase 1A Antibody (supplied by: Log in to see )

Antigen
Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Reactivity
Human, Mouse (Murine), Primate
212
121
93
21
20
9
8
6
5
4
3
2
1
1
Host
Mouse
162
47
5
Clonality
Monoclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
180
76
69
48
32
26
20
14
10
8
7
6
5
3
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN151289
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4331725 ChIP ELISA IHC IHC (p) SimWes WB Mouse IgG1 Log in to see 1B2E5 0

General

Antigen Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
Reactivity Human, Mouse (Murine), Primate
(212), (121), (93), (21), (20), (9), (8), (6), (5), (4), (3), (2), (1), (1)
Host Mouse
(162), (47), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(180), (76), (69), (48), (32), (26), (20), (14), (10), (8), (7), (6), (5), (3), (1), (1)
Pubmed 10 references available
Supplier Log in to see

Product details

Target Details Application Details Handling References Images
Purification Unpurified
Immunogen Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]
Clone 1B2E5
Isotype IgG1

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name LSD1 (KDM1A Antibody Abstract)
Background Gene Symbol: KDM1A
Molecular Weight Theoretical MW: 93 kDa
Gene ID 23028
UniProt O60341
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western Blot 1:500-1:2000, Simple Western 1:500, Chromatin Immunoprecipitation, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Buffer Ascites
Buffer contains: 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Kim, Nam, Lee, Yim, Ahn, Park, Shin, Yu, Won, Bae, Kim, Baek: "PKCα-LSD1-NF-κB-Signaling Cascade Is Crucial for Epigenetic Control of the Inflammatory Response." in: Molecular cell, Vol. 69, Issue 3, pp. 398-411.e6, 2018

Lobo, Rodrigues, Antunes, Graça, Ramalho-Carvalho, Vieira, Martins, Oliveira, Jerónimo, Henrique: "High immunoexpression of Ki67, EZH2, and SMYD3 in diagnostic prostate biopsies independently predicts outcome in patients with prostate cancer." in: Urologic oncology, 2017 (Sample species: Human).

Kashyap, Ahmad, Nilsson, Helczynski, Kenna, Persson, Gudas, Mongan: "The lysine specific demethylase-1 (LSD1/KDM1A) regulates VEGF-A expression in prostate cancer." in: Molecular oncology, Vol. 7, Issue 3, pp. 555-66, 2013 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Pajtler, Weingarten, Thor, Künkele, Heukamp, Büttner, Suzuki, Miyata, Grotzer, Rieb, Sprüssel, Eggert, Schramm, Schulte: "The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma." in: Acta neuropathologica communications, Vol. 1, pp. 19, 2013 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Bennani-Baiti, Machado, Llombart-Bosch, Kovar: "Lysine-specific demethylase 1 (LSD1/KDM1A/AOF2/BHC110) is expressed and is an epigenetic drug target in chondrosarcoma, Ewing's sarcoma, osteosarcoma, and rhabdomyosarcoma." in: Human pathology, Vol. 43, Issue 8, pp. 1300-7, 2012 (Sample species: Human). Further details: Immunohistochemistry

Janzer, Lim, Fronhoffs, Niazy, Buettner, Kirfel: "Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components." in: Biochemical and biophysical research communications, Vol. 421, Issue 4, pp. 665-70, 2012 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation

Serce, Gnatzy, Steiner, Lorenzen, Kirfel, Buettner: "Elevated expression of LSD1 (Lysine-specific demethylase 1) during tumour progression from pre-invasive to invasive ductal carcinoma of the breast." in: BMC clinical pathology, Vol. 12, pp. 13, 2012 (Sample species: Human). Further details: Immunohistochemistry

Kauffman, Robinson, Downes, Powell, Lee, Scherr, Gudas, Mongan: "Role of androgen receptor and associated lysine-demethylase coregulators, LSD1 and JMJD2A, in localized and advanced human bladder cancer." in: Molecular carcinogenesis, Vol. 50, Issue 12, pp. 931-44, 2011 (Sample species: Human). Further details: Immunohistochemistry

Schildhaus, Riegel, Hartmann, Steiner, Wardelmann, Merkelbach-Bruse, Tanaka, Sonobe, Schüle, Buettner, Kirfel: "Lysine-specific demethylase 1 is highly expressed in solitary fibrous tumors, synovial sarcomas, rhabdomyosarcomas, desmoplastic small round cell tumors, and malignant peripheral nerve sheath tumors." in: Human pathology, Vol. 42, Issue 11, pp. 1667-75, 2011 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Lim, Janzer, Becker, Zimmer, Schüle, Buettner, Kirfel: "Lysine-specific demethylase 1 (LSD1) is highly expressed in ER-negative breast cancers and a biomarker predicting aggressive biology." in: Carcinogenesis, Vol. 31, Issue 3, pp. 512-20, 2010 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,ELISA,Immunohistochemistry

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody
Immunohistochemistry (IHC) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (Image 3)
Simple Western (SimWes) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) Simple Western: LSD1 Antibody (1B2E5) [ABIN151289] - Simple Western lane view shows a...
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) Western Blot: LSD1 Antibody (1B2E5) [ABIN151289] - Analysis of LSD1 expression in Hel...
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN151289) Western Blot: LSD1 Antibody (1B2E5) [ABIN151289] - Western blot analysis using LSD1 m...