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|Antigen||Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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|Specificity||This antibody detects LSD1 at N-term.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Peptide affinity chromatography|
|Immunogen||LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1.|
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|Alternative Name||AOF2 (KDM1A Antibody Abstract)|
|Background||Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.Synonyms: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1|
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling|
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ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS containing 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|