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|Antigen||Chemokine (C Motif) Ligand 1 (XCL1) Antibodies|
|Conjugate||This XCL1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-XCL1 AntibodyTarget Details XCL1 Application Details Handling Images
|Specificity||This antibody reacts with human Lymphotactin.|
|Immunogen||Highly pure (>98%) recombinant hLymphotactin|
|Plasmids, Primers & others|
Target Details XCL1Product Details anti-XCL1 Antibody Application Details Handling Images back to top
|Alternative Name||XCL1 / Lymphotactin (XCL1 Antibody Abstract)|
|Background||Synonyms: ATAC, C motif chemokine 1, Cytokine SCM-1, LTN, Lymphotaxin, SCM-1-alpha, SCYC1, Small-inducible cytokine C1, XC chemokine ligand 1|
|Pathways||Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation|
Application DetailsProduct Details anti-XCL1 Antibody Target Details XCL1 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhLymphotactin (60 ng/mL), a concentration of 1.7 - 3.0 μg/mL of this antibody is required. ELISA: To detect hLymphotactin by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLymphotactin is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-XCL1 Antibody Target Details XCL1 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|