anti-Rat (Rattus) ALAS1 antibody for Western Blotting

Recommended ALAS1 Antibody (supplied by: Log in to see )

Antigen
Aminolevulinate, delta-, Synthase 1 (ALAS1) Antibodies
  • ALAS
  • ALAS3
  • ALASH
  • MIG4
  • ALAS-N
  • Alas-1
  • Alas-h
  • ALAS-H
  • ALASN
  • ALAS1
  • wu:fb58d01
  • wu:fi12g09
  • 5'-aminolevulinate synthase 1
  • aminolevulinic acid synthase 1
  • alanyl-tRNA synthetase protein
  • aminolevulinate, delta-, synthase 1
  • ALAS1
  • Alas1
  • alas1
  • alas1.S
  • alaS1
Epitope
N-Term
7
4
4
4
3
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
70
29
29
1
1
1
1
1
1
Host
Rabbit
59
14
Clonality
Polyclonal
Conjugate
This ALAS1 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
51
16
14
13
11
10
4
4
2
2
1
1
Options
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Catalog No. ABIN2785663
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN4279106 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN631001 WB Rabbit N-Term Log in to see Polyclonal 0
3.1357021 ABIN2459736 ELISA WB Rabbit Log in to see Polyclonal 0
3.1357021 ABIN2969414 WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN5965212 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2738227 IHC IHC (p) WB Rabbit IgG AA 146-195 Log in to see Polyclonal 0
1 ABIN1421116 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN1403245 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1385781 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6291751 IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6028834 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0
1 ABIN6020588 IF/ICC IHC IP WB Mouse Log in to see 0

General

Antigen Aminolevulinate, delta-, Synthase 1 (ALAS1) Antibodies
Epitope N-Term
(7), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(70), (29), (29), (1), (1), (1), (1), (1), (1)
Host Rabbit
(59), (14)
Clonality
Polyclonal
Conjugate This ALAS1 antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(51), (16), (14), (13), (11), (10), (4), (4), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-ALAS1 Antibody

Target Details ALAS1 Application Details Handling References for anti-ALAS1 antibody (ABIN2785663) Images
Blocking Peptide Blocking peptide for this product available: ABIN973092
Sequence ETSAGPSVVS VKTDGGDPSG LLKNFQDIMQ KQRPERVSHL LQDNLPKSVS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against ALAS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ALAS1
Plasmids, Primers & others

Target Details ALAS1

Product Details anti-ALAS1 Antibody Application Details Handling References for anti-ALAS1 antibody (ABIN2785663) Images back to top
Antigen
Alternative Name ALAS1 (ALAS1 Antibody Abstract)
Background Delta-aminolevulinate synthase (ALAS, EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.
Alias Symbols: ALAS, ALAS3, ALASH, MIG4
Protein Interaction Partner: FAM127C, KLHL35, ZNF564, TEKT4, DUSP19, SNX20, MTFR2, LONRF1, ZMAT1, CDC73, TTC23, CERK, SH2D4A, EP400, C2orf42, GNL3L, CCHCR1, POLDIP2, ZFYVE26, USP20, TMSB10, ZNF175, WIPF1, UBC, TMSB4XP6, TMSB4XP2, TMSB4XP1, TMSB4X, TCEA2, PPL, BCL7A, ALAS1, PLAU, VHL,
Protein Size: 640
Molecular Weight 70 kDa
Gene ID 211
NCBI Accession NM_000688, NP_000679
UniProt P13196
Pathways Regulation of Lipid Metabolism by PPARalpha

Application Details

Product Details anti-ALAS1 Antibody Target Details ALAS1 Handling References for anti-ALAS1 antibody (ABIN2785663) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 640 AA

Restrictions For Research Use only

Handling

Product Details anti-ALAS1 Antibody Target Details ALAS1 Application Details References for anti-ALAS1 antibody (ABIN2785663) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALAS1 antibody (ABIN2785663)

Product Details anti-ALAS1 Antibody Target Details ALAS1 Application Details Handling Images back to top
Background publications

Jung, Ohl, Stephan, Rabien, Kristiansen, Radonić, Loening, Jung: "[Quantifying gene expression in prostate carcinoma. Which endogenous reference genes are suitable?]." in: Der Urologe. Ausg. A, Vol. 46, Issue 9, pp. 1083-4, 2007

Images

Product Details anti-ALAS1 Antibody Target Details ALAS1 Application Details Handling References for anti-ALAS1 antibody (ABIN2785663) back to top
Supplier Images
Western Blotting (WB) image for anti-Aminolevulinate, delta-, Synthase 1 (ALAS1) (N-Term) antibody (ABIN2785663) WB Suggested Anti-ALAS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: