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|Antigen||PR Domain Containing 1, with ZNF Domain (PRDM1) Antibodies|
|Epitope||AA 199-409 Alternatives|
|Reactivity||Human, Mouse (Murine), Pig (Porcine) Alternatives|
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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|Purification||Protein G purified|
|Immunogen||A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]|
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|Alternative Name||BLIMP1/PRDM1 (PRDM1 Antibody Abstract)|
|Background||Gene Symbol: PRDM1|
|Pathways||Regulation of Muscle Cell Differentiation|
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|Application Notes||Western Blot 1:1000, Chromatin Immunoprecipitation 1 μg antibody/ 10 μg protein, Flow Cytometry 8 μg/mL where cells are used at 2*10^6/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-FrozenThis Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for Blimp-1 Antibody Western Blot
1. Run ~20 micrograms of protein on a 10 % SDS-PAGE.
. Block nitrocellulose membrane with 5 % blotto in TBS-0.2 % Tween-20, overnight at 4 degrees Celcius.
. Incubate membrane with diluted anti-Blimp-1 (primary antibody, cat# NB 600-235) for 1 hour at RT, in 2 % blotto in TBS-0.2 % Tween-20.
. Wash blot 4 times for 10 minutes, each, at RT with TBS-0.2 % Tween-20.
. Incubate membrane with anti-mouse IgG1 conjugated to HRP (secondary antibody), diluted in 2 % blotto in TBS-0.2 % Tween-20, for 45 minutes at RT.
. Wash 4 times for 10 minutes, each, at RT with TBS-0.2 % Tween-20.
. Incubate membrane with chemiluminescence reagent as per vendor specification. Dry blot and cover with saran wrap.
. Expose to film.9.Develop image.Immunohistochemistry / ImmunofluorescencePlease refer to the following refernence:Cattoretti, G, et al. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.J Pathol. 206(1):76-86 (2005)
|Restrictions||For Research Use only|
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Tris-Glycine and 0.15M NaCl
Buffer contains: 0.1 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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|Product cited in:||
McCarthy, Kenny: "Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia." in: Scientific reports, Vol. 6, pp. 32683, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
Gosal, Dunlop, Dhaliwal, Ivanovska, Kantores, Desjardins, Connelly, McNamara, Jain, Jankov: "Rho kinase mediates right ventricular systolic dysfunction in rats with chronic neonatal pulmonary hypertension." in: American journal of respiratory cell and molecular biology, Vol. 52, Issue 6, pp. 717-27, 2015 Further details: Flow Cytometry
Jang, Mano, Aoki, Hayashi, Muto, Nambu, Takahashi, Itoh, Taketani, Nutt, Igarashi, Shimizu, Sugai: "Mitochondrial function provides instructive signals for activation-induced B-cell fates." in: Nature communications, Vol. 6, pp. 6750, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting,Flow Cytometry
Kim, Gasper, Lee, Plisch, Svaren, Suresh: "Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 3, pp. 985-95, 2014 (Sample species: Mouse (Murine)). Further details: Flow Cytometry
Severa, Islam, Waggoner, Jiang, Kim, Ryan, Kurt-Jones, Charo, Caffrey, Boyartchuk, Luster, Fitzgerald: "The transcriptional repressor BLIMP1 curbs host defenses by suppressing expression of the chemokine CCL8." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 5, pp. 2291-304, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting
Lin, Yeh, Chen, Chiou, Chu, Yen, Lin, Chang, Sytwu: "T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells." in: Clinical immunology (Orlando, Fla.), Vol. 151, Issue 2, pp. 101-13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting
Rasul, Salamon, Nagy, Leveau, Banati, Szenthe, Koroknai, Minarovits, Klein, Klein: "The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia." in: PLoS ONE, Vol. 9, Issue 8, pp. e106008, 2014 (Sample species: Human). Further details: Western Blotting
Joo, Li, Dullaers, Kim, Duluc, Upchurch, Xue, Zurawski, Le Grand, Liu, Kuroda, Zurawski, Oh: "C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses." in: Immunity, Vol. 41, Issue 4, pp. 592-604, 2014 (Sample species: Human). Further details: Flow Cytometry
Ramon, Baker, Sahler, Kim, Feldsott, Serhan, Martínez-Sobrido, Topham, Phipps: "The specialized proresolving mediator 17-HDHA enhances the antibody-mediated immune response against influenza virus: a new class of adjuvant?" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 12, pp. 6031-40, 2014 Further details: Western Blotting
Horn, Geisen, Cermak, Becker, Nakamura, Klein, Pagano, Antebi: "DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation." in: Developmental cell, Vol. 28, Issue 6, pp. 697-710, 2014 (Sample species: Human). Further details: Western Blotting
Kim, Nakasaki, Todorova, Lake, Yuan, Jamora, Xu: "p53 Induces skin aging by depleting Blimp1+ sebaceous gland cells." in: Cell death & disease, Vol. 5, pp. e1141, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Flow Cytometry,Immunofluorescence
Parlato, Bruni, Fragapane, Salerno, Marcantonio, Borghi, Tataseo, Ciccaglione, Presutti, Romagnoli, Bozzoni, Belardelli, Gabriele: "IFN-α regulates Blimp-1 expression via miR-23a and miR-125b in both monocytes-derived DC and pDC." in: PLoS ONE, Vol. 8, Issue 8, pp. e72833, 2013 (Sample species: Human). Further details: Western Blotting
Wong, Brulois, Toth, Inn, Lee, OBrien, Lee, Gao, Cesarman, Ensser, Jung: "The product of Kaposi's sarcoma-associated herpesvirus immediate early gene K4.2 regulates immunoglobulin secretion and calcium homeostasis by interacting with and inhibiting pERP1." in: Journal of virology, Vol. 87, Issue 22, pp. 12069-79, 2013 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Takata, Sato, Nakamura, Tokunaka, Miki, Yukie Kikuti, Igarashi, Ito, Harigae, Kato, Hayashi, Oka, Hoshii, Tari, Okada, Al-Kader, Mohamad, Maeda, Tanimoto, Kinoshita, Yoshino: "Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 26, Issue 1, pp. 22-31, 2013 (Sample species: Human). Further details: Immunohistochemistry
Fan, Lee, Yu, Tzen, Chou, Chang: "Upregulated hPuf-A promotes breast cancer tumorigenesis." in: Tumour biology, Vol. 34, Issue 5, pp. 2557-64, 2013
Gao, Kazama, Yonehara: "Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells." in: International immunology, Vol. 24, Issue 5, pp. 283-92, 2012
Nagy, Adori, Rasul, Heuts, Salamon, Ujvári, Madapura, Leveau, Klein, Klein: "Soluble factors produced by activated CD4+ T cells modulate EBV latency." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 5, pp. 1512-7, 2012 (Sample species: Human). Further details: Western Blotting
Altin, Goodnow, Cook: "IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 11, pp. 5478-88, 2012 (Sample species: Mouse (Murine)). Further details: Flow Cytometry
Le Gallou, Caron, Delaloy, Rossille, Tarte, Fest: "IL-2 requirement for human plasma cell generation: coupling differentiation and proliferation by enhancing MAPK-ERK signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 1, pp. 161-73, 2012 (Sample species: Human). Further details: Western Blotting
Ramon, Gao, Serhan, Phipps: "Specialized proresolving mediators enhance human B cell differentiation to antibody-secreting cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 2, pp. 1036-42, 2012 (Sample species: Human). Further details: Western Blotting
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