anti-Human AVPR1A antibody for Immunohistochemistry

Recommended AVPR1A Antibody (supplied by: Log in to see )

Antigen
Arginine Vasopressin Receptor 1A (AVPR1A) Antibodies
  • AVPR V1a
  • AVPR1
  • V1aR
  • AVPR
  • Avpr1
  • V1a
  • VT4
  • arginine vasopressin receptor 1A
  • AVPR1A
  • Avpr1a
Epitope
Cytoplasmic Domain
22
10
8
4
4
4
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
67
34
23
5
4
3
3
3
2
2
1
1
1
Host
Rabbit
57
15
13
Clonality
Polyclonal
Conjugate
This AVPR1A antibody is un-conjugated
6
6
6
3
3
3
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
77
56
6
5
1
1
Supplier
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Catalog No. ABIN1048331
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.85804 ABIN2589529 IHC IHC (p) WB Mouse IgM Log in to see 721CT25-2-1 0
1 ABIN5894217 ELISA IHC WB Goat Log in to see Polyclonal 0
1 ABIN2909139 ELISA IHC WB Rabbit IgG AA 7-52 Log in to see Polyclonal 0
1 ABIN2207267 IHC Rabbit Log in to see Polyclonal 0

General

Antigen Arginine Vasopressin Receptor 1A (AVPR1A) Antibodies
Epitope Cytoplasmic Domain
(22), (10), (8), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(67), (34), (23), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(57), (15), (13)
Clonality
Polyclonal
Conjugate This AVPR1A antibody is un-conjugated
(6), (6), (6), (3), (3), (3)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(77), (56), (6), (5), (1), (1)
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Product Details anti-AVPR1A Antibody

Target Details AVPR1A Application Details Handling Images
Brand IHC-plus™
Specificity Human AVPR1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey (94%) Marmoset, Horse, Rabbit (89%) Mouse (83%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from cytoplasmic domain of human AVPR1A. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey (94%), Marmoset, Horse, Rabbit (89%), Mouse (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details AVPR1A

Product Details anti-AVPR1A Antibody Application Details Handling Images back to top
Antigen
Alternative Name AVPR1A / V1a Receptor (AVPR1A Antibody Abstract)
Background Name/Gene ID: AVPR1A
Subfamily: Vasopressin/oxytocin
Family: GPCR

Synonyms: AVPR1A, AVPR V1a, V1a receptor, AVPR1, V1a vasopressin receptor, Vasopressin V1a receptor, V1aR
Gene ID 552
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Response to Water Deprivation, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-AVPR1A Antibody Target Details AVPR1A Handling Images back to top
Application Notes Approved: IHC, IHC-P (2.5 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-AVPR1A Antibody Target Details AVPR1A Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-AVPR1A Antibody Target Details AVPR1A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1A (AVPR1A) (Cytoplasmic Domain) antibody (ABIN1048331) Anti-AVPR1A antibody ABIN1048331 IHC staining of human kidney. Immunohistochemistry ...
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1A (AVPR1A) (Cytoplasmic Domain) antibody (ABIN1048331) Anti-AVPR1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, par...