anti-Cow (Bovine) Arginine Vasopressin Receptor 1B antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Arginine Vasopressin Receptor 1B Antibody (supplied by: Log in to see )

Antigen
Arginine Vasopressin Receptor 1B (AVPR1B) Antibodies
  • LOC443050
  • AVPR1B
  • si:ch211-125a15.3
  • AVPR3
  • V3/V1b
  • VIBR
  • VPR3
  • arginine vasopressin receptor 1B
  • arginine vasopressin receptor 1Ab
  • AVPR1B
  • avpr1ab
  • Avpr1b
Epitope
N-Term
47
16
11
11
10
7
4
4
4
2
2
2
2
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
98
55
51
5
3
3
2
2
1
1
1
1
Host
Rabbit
112
40
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
10
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
112
85
37
30
13
6
4
2
2
1
1
1
1
Supplier
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Catalog No. ABIN270844
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1048332 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Arginine Vasopressin Receptor 1B (AVPR1B) Antibodies
Epitope N-Term
(47), (16), (11), (11), (10), (7), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
(98), (55), (51), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(112), (40)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (10), (10), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(112), (85), (37), (30), (13), (6), (4), (2), (2), (1), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Specificity Human AVPR1B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purification Immunogen affinity purified
Immunogen Synthetic 17 amino acid peptide from N-terminal extracellular domain of human AVPR1B.

Target Details

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Antigen
Alternative Name Vasopressin V1b receptor (V1bR) (AVPR1B Antibody Abstract)
Background Gene Symbol: AVPR1B
Gene ID 553
UniProt P47901
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones

Application Details

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Application Notes Immunohistochemistry-Paraffin 4 μg/mL

Not for use in: Western Blot
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for AVPR1B Antibody (NLS266)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (N-Term) antibody (ABIN270844) Immunohistochemistry-Paraffin: V1b Vasopressin R/AVPR1B Antibody [NLS266] - Analysis ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (N-Term) antibody (ABIN270844) Immunohistochemistry-Paraffin: V1b Vasopressin R/AVPR1B Antibody [NLS266] - Analysis ...
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (N-Term) antibody (ABIN270844) Immunohistochemistry: V1b Vasopressin R/AVPR1B Antibody [NLS266] - Analysis of anti-A...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (N-Term) antibody (ABIN270844) Immunohistochemistry-Paraffin: V1b Vasopressin R/AVPR1B Antibody - Anti-AVPR1B antib...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (N-Term) antibody (ABIN270844) Immunohistochemistry-Paraffin: V1b Vasopressin R/AVPR1B Antibody - Anti-AVPR1B antib...