anti-Monkey Arginine Vasopressin Receptor 1B antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Arginine Vasopressin Receptor 1B Antibody (supplied by: Log in to see )

Antigen
Arginine Vasopressin Receptor 1B (AVPR1B) Antibodies
  • LOC443050
  • AVPR1B
  • si:ch211-125a15.3
  • AVPR3
  • V3/V1b
  • VIBR
  • VPR3
  • arginine vasopressin receptor 1B
  • arginine vasopressin receptor 1Ab
  • AVPR1B
  • avpr1ab
  • Avpr1b
Epitope
Cytoplasmic Domain
48
15
11
11
11
7
5
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Monkey
93
53
47
6
4
4
3
2
2
1
1
1
Host
Rabbit
106
38
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
10
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
102
74
32
29
13
4
2
1
1
1
1
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Catalog No. ABIN1048334
$ 484.00
Plus shipping costs $45.00

General

Antigen Arginine Vasopressin Receptor 1B (AVPR1B) Antibodies
Epitope Cytoplasmic Domain
(48), (15), (11), (11), (11), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(93), (53), (47), (6), (4), (4), (3), (2), (2), (1), (1), (1)
Host Rabbit
(106), (38)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (10), (10), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(102), (74), (32), (29), (13), (4), (2), (1), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human AVPR1B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bat, Pig (88%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human AVPR1B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bat, Pig (88%).

Type of Immunogen: Synthetic peptide

Target Details

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Antigen
Alternative Name AVPR1B (AVPR1B Antibody Abstract)
Background Name/Gene ID: AVPR1B
Subfamily: Vasopressin/oxytocin
Family: GPCR

Synonyms: AVPR1B, AVPR3, V1bR, AVPR V1b, AVPR V3, V1b receptor, V1b vasopressin receptor, Vasopressin V1b receptor, Vasopressin V3 receptor, VPR3
Gene ID 553
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones

Application Details

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Application Notes Approved: IHC, IHC-P (2 - 10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody (ABIN1048334) Anti-AVPR1B antibody ABIN1048334 IHC staining of human pituitary, anterior. Immunohi...