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|Antigen||Glucose-6-Phosphate Dehydrogenase (G6PD) Antibodies|
|Epitope||AA 308-320, Internal Region Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Glucose-6-Phosphate Dehydrogenase AntibodyTarget Details Glucose-6-Phosphate Dehydrogenase Application Details Handling Images
|Specificity||This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).|
Species reactivity (expected):Mouse, Rat and Dog (Canine).
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.|
|Immunogen||Synthetic Peptide from the internal region (aa 308-320) of Human G6PD. Genename: G6PD|
Target Details Glucose-6-Phosphate DehydrogenaseProduct Details anti-Glucose-6-Phosphate Dehydrogenase Antibody Application Details Handling Images back to top
|Alternative Name||G6PD (G6PD Antibody Abstract)|
|Background||Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase|
|Gene ID||2539, 9606|
|Pathways||Regulation of Systemic Arterial Blood Pressure by Hormones|
Application DetailsProduct Details anti-Glucose-6-Phosphate Dehydrogenase Antibody Target Details Glucose-6-Phosphate Dehydrogenase Handling Images back to top
Peptide ELISA: Limit dilution: 1/32000. Western Blot: Use at 0.03-0.1 μg/mL. Detects a double band of approx. 55-60 kDa band in HumanTestis, Thyroid and Tonsil lysates. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Testis shows strongcytoplasm staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Glucose-6-Phosphate Dehydrogenase Antibody Target Details Glucose-6-Phosphate Dehydrogenase Application Details Images back to top
|Buffer||Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.|