anti-Mouse (Murine) MIF antibody for Enzyme Immunoassay

Recommended MIF Antibody (supplied by: Log in to see )

Antigen
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor L homeolog
  • macrophage migration inhibitory factor
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • mif.L
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • cl405_1
  • LOC100284546
Epitope
C-Term
30
28
22
15
10
8
4
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
188
66
66
7
6
5
4
3
2
2
2
1
1
Host
Goat
114
62
28
16
4
1
Clonality
Polyclonal
Conjugate
This MIF antibody is un-conjugated
17
10
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
179
113
54
34
29
27
13
12
11
8
4
3
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN372861
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5553090 EIA WB Goat C-Term Log in to see Polyclonal 0
1 ABIN2563866 EIA ELISA WB Goat IgG C-Term Log in to see Polyclonal 0

General

Antigen Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
Epitope C-Term
(30), (28), (22), (15), (10), (8), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(188), (66), (66), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
Host Goat
(114), (62), (28), (16), (4), (1)
Clonality
Polyclonal
Conjugate This MIF antibody is un-conjugated
(17), (10), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(179), (113), (54), (34), (29), (27), (13), (12), (11), (8), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-MIF Antibody

Target Details MIF Application Details Handling Images
Specificity This antibody recognizes Macrophage Migration Inhibitory Factor (MIF).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen Synthetic peptide (C-NAANVGWNNSTFA) from C-terminus of Human MIF
Isotype IgG
Plasmids, Primers & others

Target Details MIF

Product Details anti-MIF Antibody Application Details Handling Images back to top
Antigen
Alternative Name MIF (MIF Antibody Abstract)
Background MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Gene ID 4282
NCBI Accession NP_002406
UniProt P14174
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-MIF Antibody Target Details MIF Handling Images back to top
Application Notes ELISA: 1/128000. Western blot: 0.01-0.03 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MIF Antibody Target Details MIF Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Images

Product Details anti-MIF Antibody Target Details MIF Application Details Handling back to top
Supplier Images
 image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibody (ABIN372861) anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibody
 image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibody (ABIN372861) anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibody (Image 2)