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|Antigen||Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MIF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-MIF AntibodyTarget Details MIF Application Details Handling Images
|Specificity||This antibody recognizes Macrophage migration inhibitory factor.|
Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence from the C Terminus of the protein sequence according to NP_002406.1. Genename: MIF|
Target Details MIFProduct Details anti-MIF Antibody Application Details Handling Images back to top
|Alternative Name||MIF (MIF Antibody Abstract)|
|Background||MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase|
|Molecular Weight||12.5kDa (NP_002406.1).|
|Gene ID||4282, 9606|
|Pathways||Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-MIF Antibody Target Details MIF Handling Images back to top
Peptide ELISA: 1/128000. Western Blot: 0.01 - 0.03 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MIF Antibody Target Details MIF Application Details Images back to top
|Buffer||Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|