anti-Mouse (Murine) MIF antibody for Enzyme Immunoassay

Recommended MIF Antibody (supplied by: Log in to see )

Antigen
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor L homeolog
  • macrophage migration inhibitory factor
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • mif.L
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • cl405_1
  • LOC100284546
Epitope
C-Term
30
27
22
11
10
8
4
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
180
62
61
7
6
5
4
3
2
2
2
1
1
Host
Goat
108
61
27
16
4
1
Clonality
Polyclonal
Conjugate
This MIF antibody is un-conjugated
17
10
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
170
115
41
33
31
19
13
11
10
5
4
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN5553090
$ 455.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372861 EIA IHC (p) WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN2563866 EIA ELISA WB Goat IgG C-Term Log in to see Polyclonal 0

General

Antigen Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
Epitope C-Term
(30), (27), (22), (11), (10), (8), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(180), (62), (61), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
Host Goat
(108), (61), (27), (16), (4), (1)
Clonality
Polyclonal
Conjugate This MIF antibody is un-conjugated
(17), (10), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(170), (115), (41), (33), (31), (19), (13), (11), (10), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-MIF Antibody

Target Details MIF Application Details Handling Images
Sequence C-NAANVGWNNS TFA
Specificity This antibody recognizes Macrophage migration inhibitory factor.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the C Terminus of the protein sequence according to NP_002406.1. Genename: MIF

Target Details MIF

Product Details anti-MIF Antibody Application Details Handling Images back to top
Antigen
Alternative Name MIF (MIF Antibody Abstract)
Background MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Molecular Weight 12.5kDa (NP_002406.1).
Gene ID 4282, 9606
UniProt P14174
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-MIF Antibody Target Details MIF Handling Images back to top
Application Notes Peptide ELISA: 1/128000. Western Blot: 0.01 - 0.03 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MIF Antibody Target Details MIF Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.