anti-Mouse (Murine) MIF antibody for Western Blotting

Recommended MIF Antibody (supplied by: Log in to see )

Antigen
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor L homeolog
  • macrophage migration inhibitory factor
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • mif.L
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • cl405_1
  • LOC100284546
Epitope
Center
29
26
15
15
10
8
4
4
4
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
187
59
58
7
5
5
4
2
2
2
2
1
1
Host
Rabbit
114
60
30
9
4
1
Clonality
Polyclonal
Conjugate
This MIF antibody is un-conjugated
17
10
6
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
177
110
54
30
28
27
14
12
10
8
4
3
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2854957
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.309052 ABIN4899419 CyTOF FACS WB Goat IgG AA 2-115 Log in to see Polyclonal 12
19.884825 ABIN3021945 IHC WB Rabbit IgG Log in to see Polyclonal 0
16.884825 ABIN3185564 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
11.746842 ABIN4334481 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
10.809051 ABIN6263253 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.809051 ABIN4898944 CyTOF FACS WB Rat IgM AA 1-115 Log in to see 811409 0
10.809051 ABIN1859826 ICC IHC WB Rabbit IgG AA 4-112 Log in to see Polyclonal 0
10.809051 ABIN2563866 EIA ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
10.809051 ABIN2938188 IF/ICC IHC IP WB Rabbit AA 4-112 Log in to see Polyclonal 0
7.8090506 ABIN2786654 IHC WB Rabbit Middle Region Log in to see Polyclonal 0
7.8090506 ABIN1108247 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7.8090506 ABIN1873693 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.2468424 ABIN442166 ELISA ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
7 ABIN372861 EIA IHC (p) WB Goat IgG C-Term Log in to see Polyclonal 0
4.8090506 ABIN223614 IP WB Rabbit IgG Log in to see Polyclonal 0
4.8090506 ABIN2692635 IP ELISA WB Rabbit IgG AA 2-115 Log in to see Polyclonal 0
4.8090506 ABIN5961696 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4.8090506 ABIN2989675 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
4.8090506 ABIN6568200 WB Rabbit IgG Log in to see Polyclonal 0
4.8090506 ABIN2957668 WB Rabbit Log in to see Polyclonal 0

General

Antigen Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
Epitope Center
(29), (26), (15), (15), (10), (8), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(187), (59), (58), (7), (5), (5), (4), (2), (2), (2), (2), (1), (1)
Host Rabbit
(114), (60), (30), (9), (4), (1)
Clonality
Polyclonal
Conjugate This MIF antibody is un-conjugated
(17), (10), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(177), (110), (54), (30), (28), (27), (14), (12), (10), (8), (4), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-MIF Antibody

Target Details MIF Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to MIF (macrophage migration inhibitory factor (glycosylation-inhibiting factor))
MIF antibody [N1C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details MIF

Product Details anti-MIF Antibody Application Details Handling Images back to top
Antigen
Alternative Name MIF (MIF Antibody Abstract)
Background This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Molecular Weight 12 kDa
Gene ID 4282
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-MIF Antibody Target Details MIF Handling Images back to top
Application Notes Suggested dilution Reference ELISA 1:1000-1:10000* ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceELISA1:1000-1:10000* ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-MIF Antibody Target Details MIF Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MIF Antibody Target Details MIF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Center) antibody (ABIN2854957) WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE antibody dilute...
ELISA image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Center) antibody (ABIN2854957) ELISA Image ELISA detection of MIF using for capture at a concentration of 5 µg/mL a...
Immunofluorescence (IF) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Center) antibody (ABIN2854957) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using MIF, antibody ...
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Center) antibody (ABIN2854957) WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 15% SDS PAGE antibody diluted ...
Immunohistochemistry (IHC) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Center) antibody (ABIN2854957) IHC-P Image Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ...