anti-Rat (Rattus) MIF antibody for Western Blotting

Recommended MIF Antibody (supplied by: Log in to see )

Antigen
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor L homeolog
  • macrophage migration inhibitory factor
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • mif.L
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • cl405_1
  • LOC100284546
Epitope
AA 2-115
29
26
15
14
10
8
4
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
187
58
58
7
5
5
4
2
2
2
2
1
1
Host
Goat
115
60
29
9
4
1
Clonality
Polyclonal
Conjugate
This MIF antibody is un-conjugated
17
10
6
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
177
111
54
31
28
26
14
13
11
8
4
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4899419
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.063175 ABIN3021945 IHC WB Rabbit IgG Log in to see Polyclonal 0
17.063175 ABIN3185564 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
13.25679 ABIN1689908 IHC (p) WB Rabbit Log in to see Polyclonal 0
12.281914 ABIN4334481 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
10.806385 ABIN6263253 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.806385 ABIN4898944 CyTOF FACS WB Rat IgM AA 1-115 Log in to see 811409 0
10.806385 ABIN2563866 EIA ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
7.8063846 ABIN2786654 IHC WB Rabbit Middle Region Log in to see Polyclonal 0
7.8063846 ABIN1108247 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7.8063846 ABIN1859825 ICC IHC WB Rabbit IgG AA 4-112 Log in to see Polyclonal 0
7.8063846 ABIN1873693 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.8063846 ABIN2938189 IF/ICC IHC IP WB Rabbit AA 4-112 Log in to see Polyclonal 0
7 ABIN372861 EIA IHC (p) WB Goat IgG C-Term Log in to see Polyclonal 0
7 ABIN1493110 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
4.8063846 ABIN223614 IP WB Rabbit IgG Log in to see Polyclonal 0
4.8063846 ABIN3044495 IHC (p) WB Rabbit IgG AA 98-115, C-Term Log in to see Polyclonal 0
4.8063846 ABIN5961696 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4.8063846 ABIN2957668 WB Rabbit Log in to see Polyclonal 0
4.8063846 ABIN3031673 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN575995 WB Rabbit AA 35-84 Log in to see Polyclonal 0

General

Antigen Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) Antibodies
Epitope AA 2-115
(29), (26), (15), (14), (10), (8), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(187), (58), (58), (7), (5), (5), (4), (2), (2), (2), (2), (1), (1)
Host Goat
(115), (60), (29), (9), (4), (1)
Clonality
Polyclonal
Conjugate This MIF antibody is un-conjugated
(17), (10), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
(177), (111), (54), (31), (28), (26), (14), (13), (11), (8), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-MIF Antibody

Target Details MIF Application Details Handling References for anti-MIF antibody (ABIN4899419) Images
Specificity Detects human, mouse, and rat MIF in Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human MIF, Pro2-Ala115, Accession # AAA36315
Isotype IgG
Plasmids, Primers & others

Target Details MIF

Product Details anti-MIF Antibody Application Details Handling References for anti-MIF antibody (ABIN4899419) Images back to top
Antigen
Alternative Name MIF (MIF Antibody Abstract)
Background Alternative Names: EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene ID 4282
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-MIF Antibody Target Details MIF Handling References for anti-MIF antibody (ABIN4899419) Images back to top
Application Notes Western Blot 0.5 μg/mL
Intracellular Staining by Flow Cytometry 0.25 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-MIF Antibody Target Details MIF Application Details References for anti-MIF antibody (ABIN4899419) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-MIF antibody (ABIN4899419)

Product Details anti-MIF Antibody Target Details MIF Application Details Handling Images back to top
Product cited in:

Jalili, Horecka, Swartz, Davis, Persson: "Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 5, pp. E925-E933, 2018 Method employed by authors: Affinity Measurement (AM)

Lang, Cheng, Rolfe, Hammack, Vera, Kyle, Wang, Meissner, Ren, Cowan, Tang: "An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection." in: Stem cell reports, Vol. 11, Issue 2, pp. 348-362, 2018 Method employed by authors: Neutralization (Neut) (Sample species: Human).

DiCosmo-Ponticello, Hoover, Coffman, Cohen, Cohen: "MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells." in: Cytokine, Vol. 69, Issue 1, pp. 47-55, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Fonsato, Collino, Herrera, Cavallari, Deregibus, Cisterna, Bruno, Romagnoli, Salizzoni, Tetta, Camussi: "Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 9, pp. 1985-98, 2012 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

de Oliveira Gomes, de Oliveira Silva, Silva, de Freitas Barbosa, Franco, Angeloni, Fermino, Roque-Barreira, Bechi, Paulesu, Dos Santos, Mineo, Ferro: "Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age." in: The American journal of pathology, Vol. 178, Issue 6, pp. 2792-801, 2011 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Herrero, Nelson, Srikiatkhachorn, Gu, Anantapreecha, Fingerle-Rowson, Bucala, Morand, Santos, Mahalingam: "Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 29, pp. 12048-53, 2011 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Ansa-Addo, Lange, Stratton, Antwi-Baffour, Cestari, Ramirez, McCrossan, Inal: "Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 9, pp. 5236-46, 2010 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Meyer-Siegler, Cox, Leng, Bucala, Vera: "Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation." in: Cancer letters, Vol. 290, Issue 1, pp. 49-57, 2010 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Gilliver, Ruckshanthi, Hardman, Nakayama, Ashcroft: "Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor." in: Endocrinology, Vol. 149, Issue 11, pp. 5747-57, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Daryadel, Grifone, Simon, Yousefi: "Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha." in: The Journal of biological chemistry, Vol. 281, Issue 37, pp. 27653-61, 2006 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Paulesu, Cateni, Romagnoli, Ietta, Dantzer: "Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation." in: Biology of reproduction, Vol. 72, Issue 4, pp. 949-53, 2005 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Chuang, Hung, Tsai, Yeh, Chuang et al.: "High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic ..." in: Critical care medicine, Vol. 32, Issue 3, pp. 734-9, 2004 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Human).

Images

Product Details anti-MIF Antibody Target Details MIF Application Details Handling References for anti-MIF antibody (ABIN4899419) back to top
Supplier Images
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibody (ABIN4899419) Detection of Human MIF by Western Blot. Western blot shows lysates of THP-1 human acu...
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibody (ABIN4899419) Detection of Mouse and Rat MIF by Western Blot. Western blot shows lysates of J774A.1...