anti-Zebrafish (Danio rerio) NOX1 antibody for Western Blotting

Recommended NOX1 Antibody (supplied by: Log in to see )

Antigen
NADPH Oxidase 1 (NOX1) Antibodies
  • GP91-2
  • MOX1
  • NOH-1
  • NOH1
  • NOX1a
  • NOX1alpha
  • Nox-1
  • Nox1
  • NADPH oxidase 1
  • NOX1
  • Nox1
  • nox1
Epitope
C-Term
15
13
12
10
8
7
5
5
4
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
104
34
30
5
3
2
1
1
1
1
1
1
Host
Rabbit
101
10
5
Clonality
Polyclonal
Conjugate
This NOX1 antibody is un-conjugated
5
5
4
3
3
3
Application
Western Blotting (WB)
107
67
29
22
12
11
5
5
2
1
1
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Catalog No. ABIN2782754
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN321158 WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen NADPH Oxidase 1 (NOX1) Antibodies
Epitope C-Term
(15), (13), (12), (10), (8), (7), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(104), (34), (30), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(101), (10), (5)
Clonality
Polyclonal
Conjugate This NOX1 antibody is un-conjugated
(5), (5), (4), (3), (3), (3)
Application Western Blotting (WB)
(107), (67), (29), (22), (12), (11), (5), (5), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-NOX1 Antibody

Target Details NOX1 Application Details Handling References for anti-NOX1 antibody (ABIN2782754) Images
Blocking Peptide Blocking peptide for this product available: ABIN980821
Sequence STIATSHPKS VVGVFLCGPR TLAKSLRKCC HRYSSLDPRK VQFYFNKENF
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against NOX1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NOX1
Plasmids, Primers & others

Target Details NOX1

Product Details anti-NOX1 Antibody Application Details Handling References for anti-NOX1 antibody (ABIN2782754) Images back to top
Antigen
Alternative Name NOX1 (NOX1 Antibody Abstract)
Background Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox.Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. The NOH-1S currents were reversibly blocked by zinc, a known H+ channel inhibitor. The NOH-1S variant does not contain an electron transport chain and it is thought that H+ conductance is its main physiologic function, whereas NOH-1L may conduct H+ ions as part of its electron transport mechanism.
Alias Symbols: GP91-2, MOX1, NOH-1, NOH1
Protein Interaction Partner: NOXA1, CYBA,
Protein Size: 564
Molecular Weight 65 kDa
Gene ID 27035
NCBI Accession NM_007052, NP_008983
UniProt Q9Y5S8
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport

Application Details

Product Details anti-NOX1 Antibody Target Details NOX1 Handling References for anti-NOX1 antibody (ABIN2782754) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 564 AA

Restrictions For Research Use only

Handling

Product Details anti-NOX1 Antibody Target Details NOX1 Application Details References for anti-NOX1 antibody (ABIN2782754) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NOX1 antibody (ABIN2782754)

Product Details anti-NOX1 Antibody Target Details NOX1 Application Details Handling Images back to top
Product cited in:

Rayegan, Dehpour, Sharifi: "Studying neuroprotective effect of Atorvastatin as a small molecule drug on high glucose-induced neurotoxicity in undifferentiated PC12 cells: role of NADPH oxidase." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 41-49, 2016

Kuwano, Kawahara, Yamamoto, Teshima-Kondo, Tominaga, Masuda, Kishi, Morita, Rokutan: "Interferon-gamma activates transcription of NADPH oxidase 1 gene and upregulates production of superoxide anion by human large intestinal epithelial cells." in: American journal of physiology. Cell physiology, Vol. 290, Issue 2, pp. C433-43, 2006

Images

Product Details anti-NOX1 Antibody Target Details NOX1 Application Details Handling References for anti-NOX1 antibody (ABIN2782754) back to top
Supplier Images
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (ABIN2782754) WB Suggested Anti-NOX1 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat ce...
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (ABIN2782754) anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (ABIN2782754) NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenoc...
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (ABIN2782754) Host: Rabbit Target Name: NOX1 Sample Tissue: Human MCF7 Whole Cell Antibody Di...
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (C-Term) antibody (ABIN2782754) Host: Rabbit Target Name: NOX1 Sample Tissue: Human Jurkat Whole Cell Antibody ...