anti-Guinea Pig TACR1 antibody for Immunohistochemistry

Recommended TACR1 Antibody (supplied by: Log in to see )

Antigen
Tachykinin Receptor 1 (TACR1) Antibodies
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • tachykinin receptor 1
  • TACR1
  • Tacr1
Epitope
C-Term
23
18
15
14
11
8
7
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Rabbit, Rat (Rattus)
151
71
65
10
10
5
5
5
3
3
2
1
1
1
1
1
Host
Rabbit
137
16
9
Clonality
Polyclonal
Conjugate
This TACR1 antibody is un-conjugated
8
8
7
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
110
59
58
32
27
20
18
13
6
5
2
2
1
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Catalog No. ABIN1049098
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN1737300 FACS IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN307532 IHC IHC (fro) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN307533 ICC IHC IHC (fro) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN326021 IHC IHC (fro) Rabbit IgG AA 385-407 Log in to see Polyclonal 0
1 ABIN307534 IHC IHC (fro) Rabbit IgG Log in to see Polyclonal 0

General

Antigen Tachykinin Receptor 1 (TACR1) Antibodies
Epitope C-Term
(23), (18), (15), (14), (11), (8), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Rabbit, Rat (Rattus)
(151), (71), (65), (10), (10), (5), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(137), (16), (9)
Clonality
Polyclonal
Conjugate This TACR1 antibody is un-conjugated
(8), (8), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(110), (59), (58), (32), (27), (20), (18), (13), (6), (5), (2), (2), (1)
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Product Details anti-TACR1 Antibody

Target Details TACR1 Application Details Handling Images
Brand IHC-plus™
Specificity Human Neurokinin 1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DTNB (44 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%) Mouse, Dog, Elephant (94%) Marmoset, Bat, Bovine (89%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from C-terminus of human Neurokinin 1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%), Mouse, Dog, Elephant (94%), Marmoset, Bat, Bovine (89%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details TACR1

Product Details anti-TACR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TACR1 / NK1R (TACR1 Antibody Abstract)
Background Name/Gene ID: TACR1
Subfamily: Tachykinin
Family: GPCR

Synonyms: TACR1, Neurokinin receptor 1, NK-1R, Nk1 tachykinin receptor, NK1R, TAC1R, Tachykinin 1 receptor, NKIR, Substance-P receptor, Neurokinin 1 receptor, NK-1 receptor, Tachykinin receptor 1
Gene ID 6869
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration

Application Details

Product Details anti-TACR1 Antibody Target Details TACR1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (1 - 40 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN1049098) Anti-Neurokinin 1 Receptor / NK1R antibody IHC staining of human heart. Immunohistoch...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN1049098) Anti-Neurokinin 1 Receptor / NK1R antibody IHC staining of human spleen. Immunohistoc...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN1049098) Anti-Neurokinin 1 Receptor / NK1R antibody IHC of human heart. Immunohistochemistry o...