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|Antigen||Tachykinin Receptor 1 (TACR1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TACR1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|39 references available|
Product Details anti-TACR1 AntibodyTarget Details TACR1 Application Details Handling References for anti-TACR1 antibody (ABIN152468) Images
|Purification||Immunogen affinity purified|
|Immunogen||A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor [UniProt# P25103]|
|Plasmids, Primers & others|
Target Details TACR1Product Details anti-TACR1 Antibody Application Details Handling References for anti-TACR1 antibody (ABIN152468) Images back to top
|Alternative Name||NK1R (TACR1 Antibody Abstract)|
|Background||Gene Symbol: TACR1|
|Molecular Weight||Theoretical MW: 46 kDa|
|Pathways||Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-TACR1 Antibody Target Details TACR1 Handling References for anti-TACR1 antibody (ABIN152468) Images back to top
|Application Notes||Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 10 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 10 μg/mLThis Neurokinin 1 Receptor Antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for Neurokinin 1 Receptor Antibody Western Analysis1) Separate 50 µg of whole cell or membrane protein on a 4-20 % Tris-glycine gel.
2) Transfer the proteins from the gel onto a nitrocellulose membrane.
) Incubate the nitrocellulose membrane in Blocking solution [5 % non-fat dry milk / PBS] overnight at 4 degrees C.
) Incubate the membrane with a 1:1,000 dilution of anti-NK-1 antibody (Novus Biologicals, Inc, #NB300-119) in PBST-NFDM [PBS / 0.1 % Tween-20 / 5 % non-fat dry milk] for 2 hours at RT.
) Wash the membrane with PBST-NFDM.
) Incubate the membrane with HRP-conjugated goat anti-rabbit IgG diluted with PBST-NFDM for 1 hour at RT.
) Detect antibody with an enhanced chemiluminescence reaction (ECL Western Blotting detection kit, Pharmacia).FACS Analysis
1. Seed cells into small (T-25) flask. Add 5-10 mL media. Allow cells to grow to 80-100 % confluence.
. When flasks are confluent, empty media and add new media containing appropriate drug, hormone, or cytokine dose.
. Allow flasks to incubate for 24 hours. After incubation period, remove 2 mL of media and place 1 mL into one of two eppendorf tubes.
. Empty off remaining media. Wash with 5 mL 1X PBS.
. Add 2 mL PBS with azide (1 % Goat serum).
. Scrape cells using a cell scraper.
. Transfer cells to appropriate labeled FACSCAN tubes. Vortex all tubes
. Incubate on ice for - hour in the PBS with azide (1 % Goat serum). Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Empty off media.
. Resuspend in 1 mL of a 1:3,000 primary antibody solution [1 µL antibody per 3 mL PBS with azide (1 % Goat serum)].
. Incubate on ice for 1 hour. Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Aspirate (or decant) supernatant.
. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant and place tubes on ice.
. Resuspend in 1 mL of a 1:500 secondary antibody solution [2 µL antibody per 1 mL PBS with azide (1 % Goat serum)].
. Vortex all tubes.
. Cover with foil. Incubate for 30 minutes on ice.
. Vortex after incubation. Centrifuge at 1400 RPM for 5 minutes.
. Aspirate (or decant) supernatant. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant. Resuspend in 2 % paraformaldehyde.
. Read on flow.FACS Analysis procedure courtesy of: Rosalyn Blumenthal, Ph.D. Director Tumor Biology Garden State Cancer Center Belleville, NJ 07109IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TACR1 Antibody Target Details TACR1 Application Details References for anti-TACR1 antibody (ABIN152468) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-TACR1 antibody (ABIN152468)Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling Images back to top
|Product cited in:||
Li, Wu, Yang, Zhang, He, Xu, Zhang, Tao, Luo: "Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice." in: The international journal of biochemistry & cell biology, Vol. 107, pp. 154-165, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).
Wang, Tawfik, Corder, Low, François, Basbaum, Scherrer: "Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits." in: Neuron, Vol. 98, Issue 1, pp. 90-108.e5, 2018
Bubak, Como, Blackmon, Frietze, Mescher, Jones, Cohrs, Paucek, Baird, Nagel: "Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes." in: The Journal of infectious diseases, Vol. 218, Issue 8, pp. 1324-1335, 2018
Zhang, Zheng, Xie, Chai, Wang, Zhang, He: "Up-regulated expression of substance P in CD8(+) T cells and NK1R on monocytes of atopic dermatitis." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 93, 2017 (Sample species: Mouse (Murine)).
Henssen, Odersky, Szymansky, Seiler, Althoff, Beckers, Speleman, Schäfers, De Preter, Astrahanseff, Struck, Schramm, Eggert, Bergmann, Schulte: "Targeting tachykinin receptors in neuroblastoma." in: Oncotarget, Vol. 8, Issue 1, pp. 430-443, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Zhou, Zuo, Wang, Zhang, Yu, Yang, Yao: "Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 19, Issue 9, pp. 676-91, 2014 (Sample species: Human). Further details: Western Blotting
Fujisawa, Kashiwakura, Kita, Kikukawa, Fujitani, Sasaki-Sakamoto, Kuroda, Nunomura, Hayama, Terui, Ra, Okayama: "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 3, pp. 622-633.e9, 2014 (Sample species: Human). Further details: Flow Cytometry
Schwartz, Spitsin, Meshki, Tuluc, Douglas, Wolfe: "Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor." in: Journal of neurovirology, Vol. 19, Issue 3, pp. 219-27, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
Zhou, Zhao, Xiong, Chen, Zhang, Yu, Yang, Yao: "Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer." in: Breast cancer research and treatment, Vol. 140, Issue 1, pp. 49-61, 2013 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Altawil, Lyström, El-Nour: "Kinetics of neuronal contribution during the development of a contact allergic reaction." in: Archives of dermatological research, Vol. 304, Issue 4, pp. 273-81, 2012 (Sample species: Human). Further details: Immunohistochemistry
Humes, Simpson, Smith, Sutton, Zaitoun, Bush, Bennett, Scholefield, Spiller: "Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 24, Issue 4, pp. 318-e163, 2012 (Sample species: Human). Further details: Immunohistochemistry
Janssen, Yasuda, Vicini: "GABA(A) Receptor β3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons." in: Frontiers in cellular neuroscience, Vol. 5, pp. 15, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting
Nayate, Moore, Weiss, Taktakishvili, Lin, Talman: "Cardiac damage after lesions of the nucleus tractus solitarii." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 296, Issue 2, pp. R272-9, 2009 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry
Schmidtko, Gao, König, Heine, Motterlini, Ruth, Schlossmann, Koesling, Niederberger, Tegeder, Friebe, Geisslinger: "cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 34, pp. 8568-76, 2008 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Grimsholm, Rantapää-Dahlqvist, Dalén, Forsgren et al.: "Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the ..." in: Neuropeptides, Vol. 42, Issue 2, pp. 133-45, 2008 (Sample species: Human).
Andersson, Danielson, Alfredson, Forsgren: "Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon." in: Regulatory peptides, Vol. 150, Issue 1-3, pp. 81-7, 2008 (Sample species: Human).
Massouh, Wallman, Pourcher, Parent: "The fate of the large striatal interneurons expressing calretinin in Huntington's disease." in: Neuroscience research, Vol. 62, Issue 4, pp. 216-24, 2008 (Sample species: Human).
Hazlett, McClellan, Barrett, Liu, Zhang, Lighvani: "Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection." in: Investigative ophthalmology & visual science, Vol. 48, Issue 2, pp. 797-807, 2007 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Andersson, Danielson, Alfredson, Forsgren: "Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 15, Issue 10, pp. 1272-9, 2007 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)
Ferguson, Hardy, Werner, Hileman, Delorey, Homanics: "New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout." in: BMC neuroscience, Vol. 8, pp. 85, 2007 (Sample species: Mouse (Murine)). Further details: Western Blotting
ImagesProduct Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling References for anti-TACR1 antibody (ABIN152468) back to top