anti-Human RBBP6 antibody for Western Blotting

Recommended RBBP6 Antibody (supplied by: Log in to see )

Antigen
Retinoblastoma Binding Protein 6 (RBBP6) Antibodies
  • MY038
  • P2P-R
  • PACT
  • RBQ-1
  • SNAMA
  • 4933422O15Rik
  • AI316869
  • BB233631
  • C030034J04Rik
  • C77662
  • rbbp6
  • MGC75882
  • RBBP6
  • im:7158869
  • rbbp6l
  • RB binding protein 6, ubiquitin ligase
  • retinoblastoma binding protein 6, ubiquitin ligase
  • retinoblastoma binding protein 6
  • RBBP6
  • Rbbp6
  • rbbp6
Epitope
AA 1582-1692
25
17
16
15
5
4
3
2
2
2
1
1
1
1
Reactivity
Human
113
67
43
5
4
4
3
3
2
2
1
1
1
Host
Mouse
107
6
Clonality
Monoclonal
Conjugate
This RBBP6 antibody is un-conjugated
6
6
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
78
33
26
21
18
18
14
6
3
Options
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Available images

Catalog No. ABIN393817
$ 527.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.188007 ABIN2443899 IF IP WB Rabbit full length Log in to see Polyclonal 0
16.188007 ABIN634156 WB Rabbit N-Term Log in to see Polyclonal 0
13 ABIN562601 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 1582-1691 Log in to see 5A11 0
12.457693 ABIN6389222 ELISA WB Rabbit IgG AA 270-350 Log in to see Polyclonal 0
12.457693 ABIN5620673 WB Rabbit Center Log in to see Polyclonal 0
12.457693 ABIN2150395 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.457693 ABIN6146743 IF WB Rabbit Log in to see Polyclonal 0
7.730314 ABIN2970201 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
7.730314 ABIN6570816 IF WB Rabbit IgG Log in to see Polyclonal 0
7.730314 ABIN2564902 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
7.730314 ABIN948391 IF WB Mouse AA 1-118 Log in to see Polyclonal 0
4.730314 ABIN2774927 WB Rabbit N-Term Log in to see Polyclonal 0
4.730314 ABIN2774926 WB Rabbit N-Term Log in to see Polyclonal 0
4.730314 ABIN2706934 WB Rabbit Center Log in to see Polyclonal 0
4.730314 ABIN3198248 WB Rabbit Log in to see Polyclonal 0
4.730314 ABIN6264662 ELISA WB Rabbit IgG Log in to see Polyclonal 0
4.730314 ABIN6005994 WB Rabbit IgG full length Log in to see Polyclonal 0
4.730314 ABIN5586815 ELISA WB Rabbit Log in to see Polyclonal 0
4.730314 ABIN5586816 ELISA WB Rabbit Log in to see Polyclonal 0
4.1991267 ABIN4349510 ICC IF IHC IHC (p) IP WB Rabbit AA 1600-1650, Internal Region Log in to see Polyclonal 2

General

Antigen Retinoblastoma Binding Protein 6 (RBBP6) Antibodies
Epitope AA 1582-1692
(25), (17), (16), (15), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(113), (67), (43), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(107), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RBBP6 antibody is un-conjugated
(6), (6), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(78), (33), (26), (21), (18), (18), (14), (6), (3)
Pubmed 5 references available
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Product Details anti-RBBP6 Antibody

Target Details RBBP6 Application Details Handling References for anti-RBBP6 antibody (ABIN393817) Images
Specificity RBBP6 (NP_008841, 1582 a.a. ~ 1692 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: RBBP6 (NP_008841, 1582 a.a. ~ 1691 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 5A11
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details RBBP6

Product Details anti-RBBP6 Antibody Application Details Handling References for anti-RBBP6 antibody (ABIN393817) Images back to top
Antigen
Alternative Name RBBP6 (RBBP6 Antibody Abstract)
Background

Other Names: E3 ubiquitin-protein ligase RBBP6, 632-, Proliferation potential-related protein, Protein P2P-R, Retinoblastoma-binding Q protein 1, RBQ-1, Retinoblastoma-binding protein 6, p53-associated cellular protein of testis, RBBP6, P2PR, PACT, RBQ1

Gene Description: retinoblastoma binding protein 6

Gene Summary: The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq

Molecular Weight 201564 DA
Gene ID 5930
NCBI Accession NP_008841, NP_061173, NP_116015
UniProt Q7Z6E9
Pathways Regulatory RNA Pathways

Application Details

Product Details anti-RBBP6 Antibody Target Details RBBP6 Handling References for anti-RBBP6 antibody (ABIN393817) Images back to top
Application Notes Western blot = 1:500-1000, Immunofluorescence =10 µg/mL
Comment

Background: The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.

Restrictions For Research Use only

Handling

Product Details anti-RBBP6 Antibody Target Details RBBP6 Application Details References for anti-RBBP6 antibody (ABIN393817) Images back to top
Concentration 1 mg/mL
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-RBBP6 antibody (ABIN393817)

Product Details anti-RBBP6 Antibody Target Details RBBP6 Application Details Handling Images back to top
Background publications

Stein, Hua, Lee, Ho, Leow, Toga, Saykin, Shen, Foroud, Pankratz, Huentelman, Craig, Gerber, Allen, Corneveaux, Dechairo, Potkin, Weiner, Thompson,: "Voxelwise genome-wide association study (vGWAS)." in: NeuroImage, Vol. 53, Issue 3, pp. 1160-74, 2010

Chibi, Meyer, Skepu, G Rees, Moolman-Smook, Pugh: "RBBP6 interacts with multifunctional protein YB-1 through its RING finger domain, leading to ubiquitination and proteosomal degradation of YB-1." in: Journal of molecular biology, Vol. 384, Issue 4, pp. 908-16, 2008

Wang, Yuan, Zhou, Guo, Zhang, Kuai, Deng, Pan, Li, He: "Protein interaction data set highlighted with human Ras-MAPK/PI3K signaling pathways." in: Journal of proteome research, Vol. 7, Issue 9, pp. 3879-89, 2008

Li, Deng, Xing, Teng, Tian, Cheng, Yin, Yang, Gao, Zhu, Sun, Zhang, Yang, He: "PACT is a negative regulator of p53 and essential for cell growth and embryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 19, pp. 7951-6, 2007

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-RBBP6 Antibody Target Details RBBP6 Application Details Handling References for anti-RBBP6 antibody (ABIN393817) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) Immunoperoxidase of monoclonal antibody to RBBP6 on formalin-fixed paraffin-embedded ...
Immunohistochemistry (IHC) image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (Image 2)
Western Blotting (WB) image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunohistochemistry (IHC) image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) Immunoperoxidase of monoclonal antibody to RBBP6 on formalin-fixed paraffin-embedded ...
Immunofluorescence (IF) image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) Immunofluorescence of monoclonal antibody to RBBP6 on HeLa cell . [antibody concentra...
ELISA image for anti-Retinoblastoma Binding Protein 6 (RBBP6) (AA 1582-1692) antibody (ABIN393817) Detection limit for recombinant GST tagged RBBP6 is approximately 0.1ng/ml as a captu...