Compare & order more than 3,600 validated isotype controls. Isotype controls are negative controls that indicates the amount of background signal of the primary antibody. Isotype controls would indicate the amount of non-specific binding to the tissues or cells being examined. Antibodies raised in mice (e.g. IgG2a), for example, bind strongly to some human leukocytes regardless of the specificity of the test antibody. In this case, a mouse IgG2a isotype control would be needed with human cells or tissues to properly assess experimental results.
Why use isotype controls?
Non-specific binding of primary antibodies are typically caused by the following reasons:
- Non-specific Fc receptor binding to cells
- Non-specific antibody interactions with cellular proteins
- Cell autofluorescence
Applications for isotype controls
Isotype controls are typically used as a control in flow cytometry (FACS). An isotype control antibody can be used as a standard, blocking agent or coating protein in various scientific applications such as , , , , and .