Chromobodies® are a novel species of intracellular functional antibodies. Chromobodies® are made by fusing the antigen binding domain (VHH) derived from heavy chains from camelidae antibodies to fluorescent proteins. These fluorescent biomarkers can be used for direct detection and tracking of endogenous proteins and other cellular components in vivo. Using chromobodies ® for live cell analysis will greatly expand the quality and quantity of information.
can, in principle, detect any antigenic structure, including posttranslational modifications. Depending on the epitope chosen, can also be used to modulate protein function in living cells.
On antibodies-onlline.com, Chromobodies® are provided as which can be used to transfect individual cell lines.
A publication using the Cell Cycle (PCNA) Chromobody
Burgess A, Lorca T, Castro A (2012) Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells. PLoS ONE
7(9): e45726. doi:10.1371/journal.pone.0045726 (PLoS ONE
Some additional videos
of the ChromoTek HeLa cell line
undergoing cell division (from the laboratory of the publication authors).