anti-Zebrafish (Danio rerio) Argininosuccinate Lyase antibody for Western Blotting

Recommended Argininosuccinate Lyase Antibody (supplied by: Log in to see )

Antigen
Argininosuccinate Lyase (ASL) Antibodies
  • ASAL
  • 2510006M18Rik
  • zgc:63532
  • BA4879
  • PSPTO0125
  • argininosuccinate lyase
  • argininosuccinate lyase ArgH
  • argininosuccinate lyase L homeolog
  • ASL
  • Asl
  • asl
  • argH2
  • argH
  • arg7
  • CNC04420
  • STHERM_c13370
  • asl.L
Epitope
Middle Region
15
4
4
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
67
38
29
5
5
5
5
5
4
3
3
3
3
3
3
2
2
Host
Rabbit
56
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
40
14
13
13
9
9
7
4
4
2
2
2
1
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Catalog No. ABIN2776928
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0807028 ABIN2776927 WB Rabbit N-Term Log in to see Polyclonal 1
3.0807028 ABIN2774309 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN320664 WB Rabbit IgG AA 101-150 Log in to see Polyclonal 0
1 ABIN320665 WB Rabbit IgG AA 260-309 Log in to see Polyclonal 0
1 ABIN1100101 WB Rabbit AA 284-333 Log in to see Polyclonal 0

General

Antigen Argininosuccinate Lyase (ASL) Antibodies
Epitope Middle Region
(15), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
(67), (38), (29), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2)
Host Rabbit
(56), (11)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(40), (14), (13), (13), (9), (9), (7), (4), (4), (2), (2), (2), (1)
Pubmed 2 references available
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Product details

Target Details Application Details Handling References Images
Sequence LILYCTKEFS FVQLSDAYST GSSLMPQKKN PDSLELIRSK AGRVFGREDK
Predicted Reactivity Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against ASL. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ASL

Target Details

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Antigen
Alternative Name ASL (ASL Antibody Abstract)
Background ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: ASAL
Protein Interaction Partner: WDYHV1, ASL, GINS4, NCDN, TUFM, PDHA1, NEDD8, HK1, GDI1, BAG3, OVGP1, HMOX1, FBP1, CSNK2A2, UBC, QARS,
Protein Size: 444
Molecular Weight 49 kDa
Gene ID 435
NCBI Accession NM_001024944, NP_001020115
UniProt P04424
Pathways Response to Growth Hormone Stimulus

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 444 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, 2014

Tanaka, Nagao, Mori, Tsutsumi: "A novel stop codon mutation (X465Y) in the argininosuccinate lyase gene in a patient with argininosuccinic aciduria." in: The Tohoku journal of experimental medicine, Vol. 198, Issue 2, pp. 119-24, 2003

Images

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Supplier Images
Western Blotting (WB) image for anti-Argininosuccinate Lyase (ASL) (Middle Region) antibody (ABIN2776928) WB Suggested Anti-ASL Antibody Titration: 5.0ug/ml Positive Control: Human Liver
Western Blotting (WB) image for anti-Argininosuccinate Lyase (ASL) (Middle Region) antibody (ABIN2776928) Lanes: Lane1: 10 ug COS-7 cell lysate Primary Antibody Dilution: 1:1000 Seconda...