|Antigen||Argininosuccinate Synthase 1 (ASS1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ASS1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-ASS1 AntibodyTarget Details ASS1 Application Details Handling Images
|Specificity||This antibody detects ASS1.|
Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Cow.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from the internal region of the protein sequence according to NP_000041.2, NP_446464.1. Genename: ASS1|
|Plasmids, Primers & others|
Target Details ASS1Product Details anti-ASS1 Antibody Application Details Handling Images back to top
|Alternative Name||ASS1 (ASS1 Antibody Abstract)|
|Background||ASS1 belongs to the argininosuccinate synthase family, type 1 subfamily. It catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700].Synonyms: Argininosuccinate synthase, Citrulline-aspartate ligase|
|Molecular Weight||46.5 kDa (NP_000041.2 and NP_446464.1)|
|Gene ID||445, 9606|
|Pathways||Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-ASS1 Antibody Target Details ASS1 Handling Images back to top
Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Immunofluorescence: A customer has reported this antibody works well in IF. Immunoprecipitation: This antibody has been successfully used in immunoprecipitationassays (Corbin et al. , 2008).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ASS1 Antibody Target Details ASS1 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|