anti-Dog (Canine) ASS1 antibody for Immunofluorescence

Recommended ASS1 Antibody (supplied by: Log in to see )

Antigen
Argininosuccinate Synthase 1 (ASS1) Antibodies
  • ASS
  • CTLN1
  • AA408052
  • Ass-1
  • fold
  • ASSA
  • Ass
  • ass
  • zgc:92051
  • wu:fb95a04
  • wu:fc01e08
  • argininosuccinate synthase 1
  • argininosuccinate synthetase 1
  • argininosuccinate synthase 1 S homeolog
  • ASS1
  • Ass1
  • ass1.S
  • ass1
Epitope
Internal Region
27
11
9
8
4
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
116
30
21
13
8
7
2
2
1
1
1
Host
Goat
58
33
25
Clonality
Polyclonal
Conjugate
This ASS1 antibody is un-conjugated
6
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
106
71
56
30
28
23
22
10
5
4
1
1
Supplier
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Catalog No. ABIN5553322
$ 462.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5609721 ELISA IF IHC IP WB Biotin Goat Internal Region Log in to see Polyclonal 0
1 ABIN2561198 IF IP IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0

General

Antigen Argininosuccinate Synthase 1 (ASS1) Antibodies
Epitope Internal Region
(27), (11), (9), (8), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(116), (30), (21), (13), (8), (7), (2), (2), (1), (1), (1)
Host Goat
(58), (33), (25)
Clonality
Polyclonal
Conjugate This ASS1 antibody is un-conjugated
(6), (4), (4), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(106), (71), (56), (30), (28), (23), (22), (10), (5), (4), (1), (1)
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Product Details anti-ASS1 Antibody

Target Details ASS1 Application Details Handling Images
Sequence C-ENPKNQAPPG LYTKTQD
Specificity This antibody detects ASS1.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Cow.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from the internal region of the protein sequence according to NP_000041.2, NP_446464.1. Genename: ASS1
Plasmids, Primers & others

Target Details ASS1

Product Details anti-ASS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ASS1 (ASS1 Antibody Abstract)
Background ASS1 belongs to the argininosuccinate synthase family, type 1 subfamily. It catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700].Synonyms: Argininosuccinate synthase, Citrulline-aspartate ligase
Molecular Weight 46.5 kDa (NP_000041.2 and NP_446464.1)
Gene ID 445, 9606
UniProt P00966
Pathways Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-ASS1 Antibody Target Details ASS1 Handling Images back to top
Application Notes Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Immunofluorescence: A customer has reported this antibody works well in IF. Immunoprecipitation: This antibody has been successfully used in immunoprecipitationassays (Corbin et al. , 2008).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ASS1 Antibody Target Details ASS1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.