anti-Human PRAME antibody for Immunocytochemistry

Recommended PRAME Antibody (supplied by: Log in to see )

Antigen
Preferentially Expressed Antigen in Melanoma (PRAME) Antibodies
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
Epitope
N-Term, AA 1-100
15
13
10
7
7
3
3
2
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
75
5
4
4
4
3
2
2
1
Host
Rabbit
72
4
Clonality
Polyclonal
Conjugate
This PRAME antibody is un-conjugated
6
5
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
53
13
12
11
10
8
5
4
3
2
2
2
1
Supplier
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Available images

Catalog No. ABIN315884
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1860297 ICC IHC WB Rabbit IgG AA 416-502 Log in to see Polyclonal 0
1 ABIN1860296 ICC IHC WB Rabbit IgG AA 416-502 Log in to see Polyclonal 0
1 ABIN4239474 ICC IF FITC Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
1 ABIN4239475 ICC IF WB DyLight 405 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0

General

Antigen Preferentially Expressed Antigen in Melanoma (PRAME) Antibodies
Epitope N-Term, AA 1-100
(15), (13), (10), (7), (7), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine)
(75), (5), (4), (4), (4), (3), (2), (2), (1)
Host Rabbit
(72), (4)
Clonality
Polyclonal
Conjugate This PRAME antibody is un-conjugated
(6), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(53), (13), (12), (11), (10), (8), (5), (4), (3), (2), (2), (2), (1)
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Product Details anti-PRAME Antibody

Target Details PRAME Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an N-terminal portion of human PRAME (within residues 1-100). [Swiss-Prot# P78395]
Plasmids, Primers & others

Target Details PRAME

Product Details anti-PRAME Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRAME (PRAME Antibody Abstract)
Background Gene Symbol: PRAME
Molecular Weight Theoretical MW: 57 kDa
Gene ID 23532
UniProt P78395
Pathways Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

Application Details

Product Details anti-PRAME Antibody Target Details PRAME Handling Images back to top
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:100This PRAME antibody is useful for Western blot, where a band is seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PRAME Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PRAME primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PRAME Antibody Target Details PRAME Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product Details anti-PRAME Antibody Target Details PRAME Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-100), (N-Term) antibody (ABIN315884) Immunocytochemistry/Immunofluorescence: PRAME Antibody [NBP1-03359] - PRAME antibody ...
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-100), (N-Term) antibody (ABIN315884) Western Blot: PRAME Antibody [NBP1-03359] - Detection of PRAME in MCF7 cell lysates u...