anti-Horse (Equine) DDX3X antibody for Western Blotting

Recommended DDX3X Antibody

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) Antibodies
  • DBX
  • DDX14
  • DDX3
  • HLP2
  • D1Pas1-rs2
  • Ddx3
  • Fin14
  • An3
  • pl10
  • DDX3X
  • DEAD-box helicase 3, X-linked
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
  • DEAD-box helicase 3, X-linked L homeolog
  • DDX3X
  • Ddx3x
  • ddx3x.L
  • ddx3x
Epitope
N-Term
20
20
10
8
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
178
107
88
5
5
4
4
4
3
1
1
1
Host
Rabbit
159
19
Clonality
Polyclonal
Conjugate
This DDX3X antibody is un-conjugated
7
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
132
71
62
30
27
26
23
17
8
6
5
5
2
2
1
1
Options

Available images

Catalog No. ABIN2775180
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN6742671 WB Rabbit N-Term Polyclonal 0
1 ABIN1978345 IP WB Rabbit IgG AA 600-661, C-Term Polyclonal 0
1 ABIN1978344 IP WB Rabbit IgG AA 550-600 Polyclonal 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) Antibodies
Epitope N-Term
(20), (20), (10), (8), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(178), (107), (88), (5), (5), (4), (4), (4), (3), (1), (1), (1)
Host Rabbit
(159), (19)
Clonality
Polyclonal
Conjugate This DDX3X antibody is un-conjugated
(7), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(132), (71), (62), (30), (27), (26), (23), (17), (8), (6), (5), (5), (2), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-DDX3X Antibody

Target Details DDX3X Application Details Handling References for anti-DDX3X antibody (ABIN2775180) Images
Blocking Peptide Blocking peptide for this product available: ABIN975689
Sequence MSHVAVENAL GLDQQFAGLD LNSSDNQSGG STASKGRYIP PHLRNREATK
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against DDX3X. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human DDX3X
Plasmids, Primers & others

Target Details DDX3X

Product Details anti-DDX3X Antibody Application Details Handling References for anti-DDX3X antibody (ABIN2775180) Images back to top
Antigen
Alternative Name DDX3X (DDX3X Antibody Abstract)
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and m
Alias Symbols: DBX, DDX14, DDX3, HLP2
Protein Interaction Partner: TP53, UBC, SUMO2, SUMO3, STAU1, LGR4, MDM2, RPA3, RPA2, RPA1, EZH2, SUZ12, EED, RNF2, rev, APBB1, NEDD4, EIF3L, SND1, WBP4, EIF3D, RPS23, RPS11, RPS9, RPS7, RPS3, RPS2, PIN1, PARK2, HCVgp1, TARDBP, YWHAQ, UBD, IGSF8, NPM1, LMNA, ICAM1, CD81, SP1, EIF3K, I
Protein Size: 662
Molecular Weight 73 kDa
Gene ID 1654
NCBI Accession NM_001356, NP_001347
UniProt O00571
Pathways Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly

Application Details

Product Details anti-DDX3X Antibody Target Details DDX3X Handling References for anti-DDX3X antibody (ABIN2775180) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 662 AA

Restrictions For Research Use only

Handling

Product Details anti-DDX3X Antibody Target Details DDX3X Application Details References for anti-DDX3X antibody (ABIN2775180) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX3X antibody (ABIN2775180)

Product Details anti-DDX3X Antibody Target Details DDX3X Application Details Handling Images back to top
Background publications

Shih, Tsai, Chao, Wu Lee: "Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein." in: Oncogene, Vol. 27, Issue 5, pp. 700-14, 2008

Images

Product Details anti-DDX3X Antibody Target Details DDX3X Application Details Handling References for anti-DDX3X antibody (ABIN2775180) back to top
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) (N-Term) antibody (ABIN2775180) WB Suggested Anti-DDX3X Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) (N-Term) antibody (ABIN2775180) Sample Type: 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibo...
Immunohistochemistry (IHC) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) (N-Term) antibody (ABIN2775180) Sample Type : Human brain stem cells (NT2) Primary Antibody Dilution : 1:500 Se...