anti-Guinea Pig EEA1 antibody for Immunohistochemistry

Recommended EEA1 Antibody (supplied by: Log in to see )

Antigen
Early Endosome Antigen 1 (EEA1) Antibodies
  • MST105
  • MSTP105
  • ZFYVE2
  • im:7151048
  • si:ch211-276p10.2
  • A430109M19Rik
  • B230358H09Rik
  • early endosome antigen 1
  • early endosome antigen 1 S homeolog
  • EEA1
  • eea1
  • Eea1
  • eea1.S
Epitope
N-Term
10
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
101
56
50
12
11
10
10
9
8
5
4
4
4
4
4
4
2
2
Host
Rabbit
74
13
11
2
2
Clonality
Polyclonal
Conjugate
This EEA1 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
81
27
15
15
13
11
9
8
4
3
2
Options
Supplier
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Available images

Catalog No. ABIN2779263
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN2620243 IF IHC IHC (p) IP WB Rabbit IgG AA 175-225 Log in to see Polyclonal 0

General

Antigen Early Endosome Antigen 1 (EEA1) Antibodies
Epitope N-Term
(10), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(101), (56), (50), (12), (11), (10), (10), (9), (8), (5), (4), (4), (4), (4), (4), (4), (2), (2)
Host Rabbit
(74), (13), (11), (2), (2)
Clonality
Polyclonal
Conjugate This EEA1 antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(81), (27), (15), (15), (13), (11), (9), (8), (4), (3), (2)
Pubmed 2 references available
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Product Details anti-EEA1 Antibody

Target Details EEA1 Application Details Handling References for anti-EEA1 antibody (ABIN2779263) Images
Blocking Peptide Blocking peptide for this product available: ABIN976138
Sequence LTENLLKKEQ DYTKLEEKHN EESVSKKNIQ ATLHQKDLDC QQLQSRLSAS
Predicted Reactivity Cow: 92%, Dog: 75%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against EEA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human EEA1
Plasmids, Primers & others

Target Details EEA1

Product Details anti-EEA1 Antibody Application Details Handling References for anti-EEA1 antibody (ABIN2779263) Images back to top
Antigen
Alternative Name EEA1 (EEA1 Antibody Abstract)
Background EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Alias Symbols: MST105, ZFYVE2, MSTP105
Protein Interaction Partner: UBC, RNF2, BMI1, CFTR, ERBB2, VCP, EEA1, RAB5A, TNK2, RABEP1, RABGEF1, PDCD6IP, PXK, HDAC6, ZFYVE16, USO1, STX6, RAB22A, STX12, TOM1, RAB5C, MAPK14, RAB5B, IGF1R, NSF,
Protein Size: 1411
Molecular Weight 162 kDa
Gene ID 8411
NCBI Accession NM_003566, NP_003557
UniProt Q15075
Pathways Toll-Like Receptors Cascades

Application Details

Product Details anti-EEA1 Antibody Target Details EEA1 Handling References for anti-EEA1 antibody (ABIN2779263) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1411 AA

Restrictions For Research Use only

Handling

Product Details anti-EEA1 Antibody Target Details EEA1 Application Details References for anti-EEA1 antibody (ABIN2779263) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EEA1 antibody (ABIN2779263)

Product Details anti-EEA1 Antibody Target Details EEA1 Application Details Handling Images back to top
Product cited in:

Cebrian, Croce, Guerrero, Blanchard, Mayorga: "Rab22a controls MHC-I intracellular trafficking and antigen cross-presentation by dendritic cells." in: EMBO reports, Vol. 17, Issue 12, pp. 1753-1765, 2016

Background publications

Fratti, Chua, Deretic: "Induction of p38 mitogen-activated protein kinase reduces early endosome autoantigen 1 (EEA1) recruitment to phagosomal membranes." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46961-7, 2003

Images

Product Details anti-EEA1 Antibody Target Details EEA1 Application Details Handling References for anti-EEA1 antibody (ABIN2779263) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibody (ABIN2779263) Amount and Sample Type : 500 ug rat brain homogenate Amount of IP Antibody : 6 ug ...
Western Blotting (WB) image for anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibody (ABIN2779263) WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml ELISA Titer: 1:312500 Positi...
Immunohistochemistry (IHC) image for anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibody (ABIN2779263) Rabbit Anti-EEA1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human Adult L...