anti-Cow (Bovine) Nuclear Cap Binding Protein Subunit 1, 80kDa antibody for Western Blotting

Recommended Nuclear Cap Binding Protein Subunit 1, 80kDa Antibody

Antigen
Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
  • CBP80
  • CG7035
  • Dmel\\CG7035
  • EG:84H4.3
  • ncbp
  • sto1
  • cbp80
  • AU014645
  • AW538051
  • NCBP
  • Sto1
  • ncbp1
  • ABA HYPERSENSITIVE 1
  • ATCBP80
  • CAP-BINDING PROTEIN 80
  • ENS
  • ENSALADA
  • NUCLEAR CAP-BINDING PROTEIN CBP80
  • T10F5.8
  • T10F5_8
  • cap binding protein 80
  • nuclear cap binding protein subunit 1
  • nuclear cap binding protein subunit 1 S homeolog
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap binding protein subunit 1 L homeolog
  • ARM repeat superfamily protein
  • Nuclear cap-binding protein subunit 1-A
  • Nuclear cap-binding protein subunit 1
  • Cbp80
  • ncbp1
  • ncbp1.S
  • NCBP1
  • Ncbp1
  • ncbp1.L
  • ABH1
  • nbp1a
  • ncbp-1
Epitope
N-Term
15
12
7
3
3
3
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
76
31
27
4
3
3
2
2
2
2
1
1
1
Host
Rabbit
69
7
Clonality
Polyclonal
Conjugate
Un-conjugated
4
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
60
40
18
8
8
7
4
1
Options

Available images

Catalog No. ABIN2778796
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN6742972 WB Rabbit N-Term Polyclonal 0
1 ABIN1916275 IF IHC IHC (p) IP WB Rabbit IgG AA 1-50 Polyclonal 0
1 ABIN1916276 IF IHC IHC (p) IP WB Rabbit IgG AA 482-532 Polyclonal 0

General

Antigen Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
Epitope N-Term
(15), (12), (7), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(76), (31), (27), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(69), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(60), (40), (18), (8), (8), (7), (4), (1)
Pubmed 1 reference available
Supplier

Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN980520
Sequence SRRRHSDEND GGQPHKRRKT SDANETEDHL ESLICKVGEK SACSLESNLE
Predicted Reactivity Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 88%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against NCBP1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NCBP1

Target Details

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Antigen
Alternative Name NCBP1 (NCBP1 Antibody Abstract)
Background NCBP1 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CBP80, MGC2087, NCBP, Sto1
Protein Interaction Partner: POLDIP3, HUWE1, EIF4G1, UPF3B, EIF3B, MTOR, RPS6KB1, ALYREF, DDX39B, THOC3, THOC2, IVNS1ABP, UBC, LIN28B, LIN28A, ZNF346, SUMO1, SUZ12, EED, RNF2, BMI1, VAT1, MVP, TTC1, PPM1G, SERPINE2, NCL, NAP1L4, GFPT1, GARS, PUS1, TARDBP, YWHAE, RUVBL1, MPG, FN1, C17
Protein Size: 790
Molecular Weight 92 kDa
Gene ID 4686
NCBI Accession NM_002486, NP_002477
UniProt Q09161
Pathways Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 790 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Narita, Yung, Yamamoto, Tsuboi, Tanabe, Tanaka, Yamaguchi, Handa: "NELF interacts with CBC and participates in 3' end processing of replication-dependent histone mRNAs." in: Molecular cell, Vol. 26, Issue 3, pp. 349-65, 2007

Images

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Supplier Images
Western Blotting (WB) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) (N-Term) antibody (ABIN2778796) WB Suggested Anti-NCBP1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...